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Comparison of PCR-enzyme-linked immunosorbent assay and real-time PCR assay for diagnosis of an unusual case of cerebral toxoplasmosis in a stem cell transplant recipient.

Abstract
A PCR-enzyme-linked immunosorbent assay and a real-time PCR assay were compared for diagnosis and follow-up of cerebral toxoplasmosis in a stem cell transplant recipient. The sensitivity of detection was similar for both assays but was higher when the assays were performed on buffy coat rather than on whole blood or serum.
AuthorsJean Menotti, Gustavo Vilela, Stéphane Romand, Yves Jean-François Garin, Lionel Ades, Eliane Gluckman, Francis Derouin, Patricia Ribaud
JournalJournal of clinical microbiology (J Clin Microbiol) Vol. 41 Issue 11 Pg. 5313-6 (Nov 2003) ISSN: 0095-1137 [Print] United States
PMID14605193 (Publication Type: Case Reports, Comparative Study, Journal Article)
Chemical References
  • Antibodies, Protozoan
  • Immunoglobulin G
Topics
  • Adult
  • Animals
  • Antibodies, Protozoan (blood)
  • Enzyme-Linked Immunosorbent Assay (methods)
  • Humans
  • Immunoglobulin G (blood)
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive (therapy)
  • Male
  • Polymerase Chain Reaction (methods)
  • Stem Cell Transplantation (adverse effects)
  • Toxoplasma (classification, isolation & purification)
  • Toxoplasmosis, Cerebral (blood, diagnosis)

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