Performance of the focus and Kalon enzyme-linked immunosorbent assays for antibodies to herpes simplex virus type 2 glycoprotein G in culture-documented cases of genital herpes.

Abstract

Glycoprotein G-based herpes simplex virus type 2 (HSV-2) enzyme-linked immunosorbent assays from Focus and Kalon were performed with specimens from 118 patients with culture-documented genital herpes episodes, and their results were compared. Sensitivity was 52% by Kalon and 86% by Focus for first HSV-2 episodes and 100% (for each of the two tests) for recurrent HSV-2. Median times to seroconversion were 120 days by the Kalon assay, 21 days by the Focus assay, and 68 days by Western blotting assay. Values for specificity were 100% (Kalon) and 93% (Focus).

• Authors: Rhoda Ashley Morrow, David Friedrich, Elizabeth Krantz
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 41 Issue 11 Pg. 5212-4 (Nov 2003) ISSN: 0095-1137 [Print] United States
• PMID: 14605166 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Viral
• Viral Envelope Proteins
• glycoprotein G, herpes simplex virus type 2

Topics

• Antibodies, Viral (analysis)
• Enzyme-Linked Immunosorbent Assay (methods)
• Herpes Genitalis (immunology)
• Herpesvirus 1, Human (isolation & purification)
• Herpesvirus 2, Human (immunology, isolation & purification)
• Humans
• Time Factors
• Viral Envelope Proteins (analysis)