| Abstract | A DNA vaccine codifying the mycobacterial hsp65 can prevent infection with Mycobacterium tuberculosis in a prophylactic setting and also therapeutically reduce the number of bacteria in infected mice. The protective mechanism is thought to be related to Th1-mediated events that result in bacterial killing. To determine the best method of hsp65 introduction for vaccination efficacy against tuberculosis (TB), we evaluated the immunogenicity and protection of DNA-hsp65 administered by gene gun bombardment or intramuscular (i.m.) injection of naked DNA. Immunization by gene gun induced immune response with plasmid doses 100-fold lower than those required for intramuscular immunization. However, in contrast to intramuscular immunization, which was protective in these studies, gene gun immunization did not protect BALB/c mice against challenge infection. |
| Authors | K M Lima, S A dos Santos, R R Santos, I T Brandão, J M Rodrigues Jr, C L Silva
(Affiliation: Instituto do Milênio REDE-TB, Department of Biochemistry and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, 14049-900 Ribeirão Preto, SP, Brazil.)
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| Journal | Vaccine
(Vaccine)
Vol. 22
Issue 1
Pg. 49-56
(Dec 8 2003)
ISSN: 0264-410X Netherlands |
| PMID | 14604570
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Antibodies, Bacterial
- Bacterial Proteins
- Chaperonins
- Cytokines
- DNA, Bacterial
- Tuberculosis Vaccines
- Vaccines, DNA
- heat-shock protein 65, Mycobacterium
- Gold
|
| Topics |
- Animals
- Antibodies, Bacterial
(analysis, biosynthesis)
- Bacterial Proteins
(immunology)
- Biolistics
- Chaperonins
(immunology)
- Cytokines
(biosynthesis)
- DNA, Bacterial
(biosynthesis, genetics, immunology)
- Female
- Gold
- Injections, Intramuscular
- Mice
- Mice, Inbred BALB C
- Microspheres
- Tuberculosis Vaccines
(immunology)
- Vaccines, DNA
(immunology)
|
