Application of 99mTc labeled chimeric Fab fragments of monoclonal antibody A7 for immunoscintigraphy of pancreatic carcinoma.

Abstract

BACKGROUND:

One of the reasons for the poor prognosis of pancreatic carcinoma is the difficulty of obtaining an early diagnosis of pancreatic carcinoma. One possibility is the application of radioimmunoscintigraphy using radiolabeled monoclonal antibodies (Mabs).

METHODS:

We labeled chimeric (human/mouse) Fab fragments of Mab A7 (chA7Fab) with (99m)Tc and examined the distribution of (99m)Tc-labeled chA7Fab in nude mice bearing human pancreatic carcinoma.

RESULTS:

The tumor accumulation of (99m)Tc-labeled chA7Fab was larger than that of (99m)Tc-labeled A7 from 2 to 6 hr after injection. (99m)Tc-labeled chA7Fab disappeared from blood more rapidly than (99m)Tc-labeled A7. For all resected normal tissues except kidney, the accumulation of (99m)Tc-labeled chA7Fab was low and similar to that of (99m)Tc-labeled A7.

CONCLUSIONS:

Because the half-life of (99m)Tc is short (6 hr), chA7Fab, which accumulates rapidly in tumors, may be a better carrier of (99m)Tc than intact Mab A7.

• Authors: Eigo Otsuji, Hiroomi Matsumura, Kazuma Okamoto, Atsushi Toma, Yoshiaki Kuriu, Daisuke Ichikawa, Akeo Hagiwara, Hisakazu Yamagishi
• Journal: Journal of surgical oncology (J Surg Oncol) Vol. 84 Issue 3 Pg. 160-5 (Nov 2003) ISSN: 0022-4790 [Print] United States
• PMID: 14598360 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2003 Wiley-Liss, Inc.

Chemical References

• Antibodies, Monoclonal
• Immunoglobulin Fab Fragments
• Technetium

Topics

• Animals
• Antibodies, Monoclonal (pharmacokinetics)
• Cell Line, Tumor
• Humans
• Immunoglobulin Fab Fragments (immunology)
• Mice
• Mice, Nude
• Pancreatic Neoplasms (diagnostic imaging, metabolism)
• Radioimmunodetection
• Technetium (pharmacokinetics)
• Tissue Distribution