Right ventricle of patients undergoing congenital cardiac surgery differentially expresses haem oxygenase-1 and heat shock protein 70 genes.

Abstract

Reactive oxygen species are implicated in the pathogenesis of cardiac hypertrophy. Haem oxygenase-1 (HO-1), the rate-limiting enzyme in haem catabolism, is induced by oxidative stress and confers protection against oxidative tissue injuries. We used Northern blotting to examine expression of HO-1 and heat shock protein 70 (HSP70) in the hypertrophic cardiac muscle of eight patients (one infant and seven children) who underwent surgery for congenital heart disease. Levels of HO-1 and HSP70 mRNA were significantly increased in all specimens, but the orders of magnitude of the increases were different, suggesting that the genes expressing HO-1 and HSP70 are regulated separately.

• Authors: M Takeuchi, T Takahashi, N Taga, T Iwasaki, K Ohe, H Shimizu, T Suzuki, H Nakatsuka, M Yokoyama, S Sano, R Akagi, K Morita
• Journal: The Journal of international medical research (J Int Med Res) 2003 Sep-Oct Vol. 31 Issue 5 Pg. 413-7 ISSN: 0300-0605 [Print] England
• PMID: 14587309 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• HSP70 Heat-Shock Proteins
• Membrane Proteins
• HMOX1 protein, human
• Heme Oxygenase (Decyclizing)
• Heme Oxygenase-1

Topics

• Antioxidants (metabolism)
• Blotting, Northern
• Child, Preschool
• Female
• HSP70 Heat-Shock Proteins (biosynthesis)
• Heart Diseases (congenital)
• Heart Ventricles (enzymology)
• Heme Oxygenase (Decyclizing) (biosynthesis)
• Heme Oxygenase-1
• Humans
• Infant
• Male
• Membrane Proteins
• Oxidative Stress