Glomerular immune deposits in murine lupus models may contain histones.

Abstract

Two types of lupus mice, NZB/NZW F1 female hybrids and mice with graft-versus-host disease (GVHD), were studied. Histones H3 and H2A were detected by immunofluorescence in glomeruli of 22/22 proteinuric GVHD and 8/12 proteinuric NZB/W F1 female mice; in non-proteinuric animals, 3/5 GVHD and 2/27 NZB/W F1 female were positive. Using antibodies to histone peptides it was shown that mainly the N-terminal regions of histones H3 and H2A were exposed in glomerular deposits. Western blot analysis revealed antibodies to histone subfractions in sera of 33/34 lupus mice that developed proteinuria. This study provides evidence that histones are involved in the pathogenesis of lupus nephritis.

• Authors: T Schmiedeke, F Stoeckl, S Muller, Y Sugisaki, S Batsford, R Woitas, A Vogt
• Journal: Clinical and experimental immunology (Clin Exp Immunol) Vol. 90 Issue 3 Pg. 453-8 (Dec 1992) ISSN: 0009-9104 [Print] England
• PMID: 1458682 (Publication Type: Journal Article)

Chemical References

• Antigen-Antibody Complex
• Histones

Topics

• Animals
• Antibody Specificity
• Antigen-Antibody Complex (chemistry)
• Disease Models, Animal
• Female
• Fluorescent Antibody Technique
• Histones (analysis)
• Kidney Glomerulus (immunology)
• Lupus Nephritis (immunology)
• Male
• Mice
• Mice, Inbred C57BL
• Mice, Inbred DBA
• Mice, Inbred NZB