HMN-176 ((E)-4-[[2-N-[4-methoxybenzenesulfonyl]amino]stilbazole]1-
oxide) is an active metabolite of
HMN-214 ((E)-4-[2-[2-(N-acetyl-N-[4-methoxybenzenesulfonyl]amino)stilbazole]]1-
oxide), which has a potent antitumor activity in mouse xenograft models. In this study, we show that
HMN-176 circumvents multidrug resistance in a K2 human
ovarian cancer subline selected for
Adriamycin resistance (K2/ARS). Upon treatment of K2/ARS cells with 3 microM
HMN-176, the GI(50) of
Adriamycin for the cells decreased by approximately 50%. To explore the molecular mechanism of this effect, we assessed the expression of the multidrug resistance gene (MDR1), which is constitutive in K2/ARS cells, at both the
protein and the
mRNA level. Western and reverse transcription-PCR analysis revealed that the expression of MDR1 was significantly suppressed by treatment with
HMN-176. Furthermore, when administered p.o.,
HMN-214 suppressed the expression of MDR1
mRNA in a mouse xenograft model implanted with KB-A.1, an
Adriamycin-resistant cell line.
Luciferase reporter fusion gene analysis demonstrated that
HMN-176 inhibited the Y-box-dependent promoter activity of the MDR1 gene in a dose-dependent manner. Moreover, we show by electrophoretic mobility shift assay that
HMN-176 inhibits the binding of NF-Y, which is thought to be an essential factor for the basal expression of MDR1, to its target Y-box consensus sequence in the MDR-1 promoter. Inhibition of MDR-1 expression was achieved with pharmacological concentrations of
HMN-176, suggesting that
HMN-176 may act by two different mechanisms-cytotoxicity and MDR1 down-regulation-simultaneously. The data presented strongly suggest that the antitumor mechanism of
HMN-176 (or its
prodrug HMN-214 in vivo) is quite different from those of known
antitumor agents.