The tumorigenic characteristics of lime-piper betel quid-transformed JB6 cells.

Betel quid chewing is a general oral habit in Taiwan, India, southeastern Asian and South Africa with or without the additive of tobacco, alcohol or lime. In this study, the tumor-promoting neoplastic transformation effect of Lime-Piper betel quid (LPB) was examined on JB6 cells. The treatment of LPB at a high dose (1.0 mg/ml) for over 5 days or at lower doses (0.1, 0.5 mg/ml) for over 15 days induced the formation of transformed foci. The transformed cells showed the characteristics of colony formation in soft agar, higher growth rate and multilayer on culture dish. A two-fold induction of the protein levels of c-fos and c-jun proto-oncogenes was observed in the cells from the 50th passage (Cl1/p50, Cl2/p50 and Cl3/p50), suggesting that LPB-transformed cells were oncogenic. In addition, the LPB-transformed cells possessed an elevated level of c-Myc and an increased cell population distributed in the S phase of the cell cycle. These results demonstrated the promotion effect of LPB and indicate that it could be a tumor promoter.
AuthorsMing-Hsun Lin, Chau-Jong Wang, Hui-Pei Huang, Ming-Yung Chou, Fen-Pi Chou
JournalArchives of toxicology (Arch Toxicol) Vol. 78 Issue 3 Pg. 167-73 (Mar 2004) ISSN: 0340-5761 [Print] Germany
PMID14576975 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Alkaloids
  • Calcium Compounds
  • Carcinogens
  • Oxides
  • Plant Extracts
  • Proto-Oncogene Proteins c-fos
  • Proto-Oncogene Proteins c-jun
  • Proto-Oncogene Proteins c-myc
  • lime-piper betel quid
  • Alkaloids
  • Areca (toxicity)
  • Blotting, Western
  • Calcium Compounds (pharmacology)
  • Carcinogens (toxicity)
  • Cell Line (drug effects)
  • Cell Proliferation
  • Cell Transformation, Neoplastic (chemically induced)
  • Citrus aurantiifolia (toxicity)
  • Flow Cytometry
  • Gene Expression Regulation (drug effects)
  • Mastication
  • Oxides (pharmacology)
  • Piper
  • Piperaceae (toxicity)
  • Plant Extracts (pharmacology, toxicity)
  • Proto-Oncogene Proteins c-fos (biosynthesis, genetics)
  • Proto-Oncogene Proteins c-jun (biosynthesis, genetics)
  • Proto-Oncogene Proteins c-myc (biosynthesis, genetics)
  • S Phase (drug effects)
  • Taiwan
  • Time Factors

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: