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The tumorigenic characteristics of lime-piper betel quid-transformed JB6 cells.

Abstract
Betel quid chewing is a general oral habit in Taiwan, India, southeastern Asian and South Africa with or without the additive of tobacco, alcohol or lime. In this study, the tumor-promoting neoplastic transformation effect of Lime-Piper betel quid (LPB) was examined on JB6 cells. The treatment of LPB at a high dose (1.0 mg/ml) for over 5 days or at lower doses (0.1, 0.5 mg/ml) for over 15 days induced the formation of transformed foci. The transformed cells showed the characteristics of colony formation in soft agar, higher growth rate and multilayer on culture dish. A two-fold induction of the protein levels of c-fos and c-jun proto-oncogenes was observed in the cells from the 50th passage (Cl1/p50, Cl2/p50 and Cl3/p50), suggesting that LPB-transformed cells were oncogenic. In addition, the LPB-transformed cells possessed an elevated level of c-Myc and an increased cell population distributed in the S phase of the cell cycle. These results demonstrated the promotion effect of LPB and indicate that it could be a tumor promoter.
AuthorsMing-Hsun Lin, Chau-Jong Wang, Hui-Pei Huang, Ming-Yung Chou, Fen-Pi Chou
JournalArchives of toxicology (Arch Toxicol) Vol. 78 Issue 3 Pg. 167-73 (Mar 2004) ISSN: 0340-5761 [Print] Germany
PMID14576975 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Alkaloids
  • Calcium Compounds
  • Carcinogens
  • Oxides
  • Plant Extracts
  • Proto-Oncogene Proteins c-fos
  • Proto-Oncogene Proteins c-jun
  • Proto-Oncogene Proteins c-myc
  • lime-piper betel quid
Topics
  • Alkaloids
  • Areca (toxicity)
  • Blotting, Western
  • Calcium Compounds (pharmacology)
  • Carcinogens (toxicity)
  • Cell Line (drug effects)
  • Cell Proliferation
  • Cell Transformation, Neoplastic (chemically induced)
  • Citrus aurantiifolia (toxicity)
  • Flow Cytometry
  • Gene Expression Regulation (drug effects)
  • Mastication
  • Oxides (pharmacology)
  • Piper
  • Piperaceae (toxicity)
  • Plant Extracts (pharmacology, toxicity)
  • Proto-Oncogene Proteins c-fos (biosynthesis, genetics)
  • Proto-Oncogene Proteins c-jun (biosynthesis, genetics)
  • Proto-Oncogene Proteins c-myc (biosynthesis, genetics)
  • S Phase (drug effects)
  • Taiwan
  • Time Factors

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