Abstract |
SA-IGIV is a human polyclonal immunoglobulin containing elevated levels of antibodies specific for the fibrinogen-binding MSCRAMM protein clumping factor A (ClfA). In vitro, SA-IGIV specifically recognized ClfA that was expressed on the surface of Staphylococcus aureus and inhibited bacterial adherence to immobilized human fibrinogen by >95%. Moreover, SA-IGIV efficiently opsonized ClfA-coated fluorescent beads and facilitated phagocytosis by human polymorphonuclear leukocytes. To determine its potential therapeutic efficacy, SA-IGIV was evaluated in combination with vancomycin in a rabbit model of catheter-induced aortic valve infective endocarditis (IE) caused by methicillin-resistant S. aureus (MRSA). The combination therapy was more effective than vancomycin alone in sterilizing all valvular vegetations when used therapeutically during early (12-h) IE. The combination therapy resulted in clearance of bacteremia that was significantly faster than that of vancomycin alone in animals with well-established (24-h) IE. Therefore, in both early and well-established MRSA IE, the addition of SA-IGIV to a standard antibiotic regimen ( vancomycin) increased bacterial clearance from the bloodstream and/or vegetations.
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Authors | John Vernachio, Arnold S Bayer, Thuan Le, Yin-Li Chai, Bradley Prater, Amy Schneider, Brenda Ames, Peter Syribeys, Jeffrey Robbins, Joseph M Patti |
Journal | Antimicrobial agents and chemotherapy
(Antimicrob Agents Chemother)
Vol. 47
Issue 11
Pg. 3400-6
(Nov 2003)
ISSN: 0066-4804 [Print] United States |
PMID | 14576094
(Publication Type: Journal Article)
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Chemical References |
- Anti-Bacterial Agents
- Immunoglobulin G
- Immunoglobulins
- Opsonin Proteins
- Recombinant Proteins
- Vancomycin
- Fibrinogen
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Topics |
- Animals
- Anti-Bacterial Agents
(pharmacology)
- Bacteremia
(drug therapy, microbiology)
- Endocarditis, Bacterial
(drug therapy, microbiology)
- Female
- Fibrinogen
(metabolism)
- Flow Cytometry
- Humans
- Immunoglobulin G
(immunology)
- Immunoglobulins
(therapeutic use)
- Immunotherapy
- Methicillin Resistance
- Opsonin Proteins
(pharmacology)
- Rabbits
- Recombinant Proteins
(pharmacology)
- Staphylococcal Infections
(drug therapy, microbiology)
- Vancomycin
(pharmacology)
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