A K(+) channel activated by cholinergic muscarinic receptors in chick ciliary ganglion neurons at early developmental stage.

Abstract

Embryonic chick ciliary ganglion (CG) neurons obtained from E7-E8 ganglia maintained in serum-free medium were stimulated with 50 microM muscarine. A fast hyperpolarization of the membrane potential was observed in 25% of the cells tested, that in some cases was associated with a slower depolarization. Accordingly, in voltage clamp experiments, either an outward current or a biphasic current response could be observed. Single-channel experiments provide evidence that these signals can be associated to the activation of a K(+) channel whose conductance is 20 pS.

• Authors: Carla Distasi, Alessandra Gilardino, Jessica Erriquez, Pollyanna Zamburlin, Davide Lovisolo
• Journal: Brain research (Brain Res) Vol. 991 Issue 1-2 Pg. 262-6 (Nov 21 2003) ISSN: 0006-8993 [Print] Netherlands
• PMID: 14575902 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Muscarinic Agonists
• Potassium Channels
• Receptors, Cholinergic
• Receptors, Muscarinic
• Muscarine

Topics

• Animals
• Chick Embryo
• Ganglia, Parasympathetic (drug effects, physiology)
• Membrane Potentials (drug effects, physiology)
• Muscarine (pharmacology)
• Muscarinic Agonists (pharmacology)
• Neurons (drug effects, physiology)
• Patch-Clamp Techniques
• Potassium Channels (drug effects, physiology)
• Receptors, Cholinergic (drug effects, physiology)
• Receptors, Muscarinic (drug effects, physiology)