Bovine plasma beta-mannosidase activity and its potential use for beta-mannosidosis carrier detection.

Abstract

Plasma beta-mannosidase activities were determined for Salers cattle from 8 herds as an evaluation of this method for detection of beta-mannosidosis heterozygotes. Several biological factors, such as age, gender, herd, and risk of being a beta-mannosidosis carrier, were considered in this study. The mean enzyme activity for obligate heterozygotes (n = 8) was 55 U/ml (range = 43-65 U/ml), which was 59% of the mean enzyme activity for cattle that were low risk for being a carrier. These data indicate that bovine beta-mannosidosis is characterized by a gene dosage effect. The analytical and biological variation of plasma beta-mannosidase activity that was observed necessitates limiting the test to adult fullblood/purebred Salers cattle within a herd. Plasma beta-mannosidase analysis provides important information for intraherd selection of Salers cattle that are heterozygous for beta-mannosidosis.

• Authors: K T Cavanagh, M Z Jones, B Abbitt, R Skinner
• Journal: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc (J Vet Diagn Invest) Vol. 4 Issue 4 Pg. 434-40 (Oct 1992) ISSN: 1040-6387 [Print] United States
• PMID: 1457547 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Mannosidases
• beta-Mannosidase

Topics

• Aging
• Analysis of Variance
• Animals
• Cattle (abnormalities, blood)
• Female
• Genetic Carrier Screening (methods)
• Male
• Mannosidases (blood)
• Reproducibility of Results
• alpha-Mannosidosis (genetics, veterinary)
• beta-Mannosidase