Abstract | BACKGROUND: METHODS: RESULTS: Western blotting showed marked overexpression of caveolin-1 protein in cirrhotic liver, while caveolin-1 was almost undetectable in control liver tissue. Endothelial NOS was expressed at a slightly higher level in cirrhotic liver than in control liver tissue. Calmodulin was expressed abundantly in control liver tissue and at a low level in cirrhotic liver tissue. By ISH, eNOS mRNA was localized on portal vein and hepatic lining cells, and caveolin-1 mRNA was almost undetectable in normal liver tissue. In cirrhotic liver tissue, caveolin-1 mRNA was overexpressed on hepatic sinusoidal lining cells, while eNOS mRNA expression was similar to that in normal liver. CONCLUSIONS: Enhanced caveolin-1 expression may be associated with a significant reduction in NO catalytic activity in cirrhosis.
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Authors | Hiroaki Yokomori, Masaya Oda, Kazunori Yoshimura, Masahiko Nomura, Go Wakabayashi, Masaki Kitajima, Hiromasa Ishii |
Journal | Journal of gastroenterology
(J Gastroenterol)
Vol. 38
Issue 9
Pg. 854-60
( 2003)
ISSN: 0944-1174 [Print] Japan |
PMID | 14564631
(Publication Type: Journal Article)
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Chemical References |
- CAV1 protein, human
- Calmodulin
- Caveolin 1
- Caveolins
- RNA, Messenger
- Nitric Oxide
- NOS3 protein, human
- Nitric Oxide Synthase
- Nitric Oxide Synthase Type III
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Topics |
- Aged
- Blotting, Western
- Calmodulin
(metabolism)
- Carcinoma, Hepatocellular
(complications, metabolism)
- Caveolin 1
- Caveolins
(genetics, metabolism)
- Female
- Hepatitis C
(complications, metabolism)
- Humans
- In Situ Hybridization
- Liver Cirrhosis
(etiology, metabolism)
- Liver Neoplasms
(complications, metabolism)
- Male
- Middle Aged
- Nitric Oxide
(metabolism)
- Nitric Oxide Synthase
(genetics, metabolism)
- Nitric Oxide Synthase Type III
- RNA, Messenger
(genetics)
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