Heterozygous abnormal fibrinogen Osaka III with the replacement of gamma arginine-275 by histidine has an apparently higher molecular weight gamma-chain variant.

Congenitally abnormal fibrinogen Osaka III with the replacement of gamma Arg-275 by His was found in a 38-year-old female with no bleeding or thrombotic tendency. Release of fibrinopeptide(s) by thrombin or reptilase was normal, but her thrombin or reptilase time in the absence of calcium was markedly prolonged and the polymerization of preformed fibrin monomer which was prepared by the treatment of fibrinogen with thrombin or reptilase was also markedly defective. Propositus' fibrinogen had normal crosslinking abilities of alpha- and gamma-chains. Analysis of fibrinogen chains on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in the system of Laemmli only revealed the presence of abnormal gamma-chain with an apparently higher molecular weight, the presence of which was more clearly detected with SDS-PAGE of fibrin monomer obtained by thrombin treatment. Purified fragment D1 of fibrinogen Osaka III also seemed to contain an apparently higher molecular weight fragment D1 gamma remnant on Laemmli gels, which was digested faster than the normal control by plasmin in the presence of [ethylenebis(oxyethylenenitrilo)]tetraacetic acid (EGTA).
AuthorsN Yoshida, S Imaoka, H Hirata, M Matsuda, S Asakura
JournalThrombosis and haemostasis (Thromb Haemost) Vol. 68 Issue 5 Pg. 534-8 (Nov 10 1992) ISSN: 0340-6245 [Print] GERMANY
PMID1455400 (Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Fibrin Fibrinogen Degradation Products
  • Fibrinogens, Abnormal
  • fibrinogen D fragment
  • fibrinogen Kawaguchi
  • Adult
  • Amino Acid Sequence
  • Female
  • Fibrin Fibrinogen Degradation Products (chemistry, genetics, isolation & purification)
  • Fibrinogens, Abnormal (chemistry, genetics, isolation & purification)
  • Heterozygote
  • Humans
  • Molecular Sequence Data
  • Molecular Weight
  • Protein Conformation

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