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Dual effect of saikogenin D: in vitro inhibition of prostaglandin E2 production and elevation of intracellular free Ca2+ concentration in C6 rat glioma cells.

Abstract
To clarify the pharmacological profile of saikogenin D, we examined the effect of saikogenin D on prostaglandin E2 (PGE2) production and intracellular free Ca2+ concentration ([Ca2+]i) in C6 rat glioma cells. Saikogenin D (1-20 microM) inhibited PGE2 production induced by the Ca2+ ionophore A23187 in a concentration-dependent manner with the IC50 of about 3 microM. Saikogenin D did not affect the conversion of arachidonic acid into PGE2 in microsomal preparations. On the other hand, saikogenin D elevated [Ca2+]i in a concentration-dependent manner (10-100 microM) with the EC50 value of about 35 microM in the presence or absence of extracellular Ca2+. These results suggest that saikogenin D possesses a dual effect: an inhibition of A23187-induced PGE2 production without a direct inhibition of cyclooxygenase activity; and an elevation of [Ca2+]i that is attributed to Ca2+ release from intracellular stores.
AuthorsYasuo Kodama, Lv Xiaochuan, Chikako Tsuchiya, Yasushi Ohizumi, Makoto Yoshida, Norimichi Nakahata
JournalPlanta medica (Planta Med) Vol. 69 Issue 8 Pg. 765-7 (Aug 2003) ISSN: 0032-0943 [Print] Germany
PMID14531029 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Anti-Inflammatory Agents, Non-Steroidal
  • Prostaglandin Antagonists
  • saikogenin A
  • Oleanolic Acid
  • Dinoprostone
  • Calcium
Topics
  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal (administration & dosage, pharmacology, therapeutic use)
  • Bupleurum
  • Calcium (metabolism)
  • Cell Line, Tumor (drug effects)
  • Dinoprostone (antagonists & inhibitors, biosynthesis)
  • Dose-Response Relationship, Drug
  • Glioma (drug therapy, pathology)
  • Oleanolic Acid (administration & dosage, analogs & derivatives, pharmacology, therapeutic use)
  • Phytotherapy
  • Plant Roots
  • Prostaglandin Antagonists (administration & dosage, pharmacology, therapeutic use)
  • Rats

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