Abstract |
To clarify the pharmacological profile of saikogenin D, we examined the effect of saikogenin D on prostaglandin E2 ( PGE2) production and intracellular free Ca2+ concentration ([Ca2+]i) in C6 rat glioma cells. Saikogenin D (1-20 microM) inhibited PGE2 production induced by the Ca2+ ionophore A23187 in a concentration-dependent manner with the IC50 of about 3 microM. Saikogenin D did not affect the conversion of arachidonic acid into PGE2 in microsomal preparations. On the other hand, saikogenin D elevated [Ca2+]i in a concentration-dependent manner (10-100 microM) with the EC50 value of about 35 microM in the presence or absence of extracellular Ca2+. These results suggest that saikogenin D possesses a dual effect: an inhibition of A23187-induced PGE2 production without a direct inhibition of cyclooxygenase activity; and an elevation of [Ca2+]i that is attributed to Ca2+ release from intracellular stores.
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Authors | Yasuo Kodama, Lv Xiaochuan, Chikako Tsuchiya, Yasushi Ohizumi, Makoto Yoshida, Norimichi Nakahata |
Journal | Planta medica
(Planta Med)
Vol. 69
Issue 8
Pg. 765-7
(Aug 2003)
ISSN: 0032-0943 [Print] Germany |
PMID | 14531029
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Inflammatory Agents, Non-Steroidal
- Prostaglandin Antagonists
- saikogenin A
- Oleanolic Acid
- Dinoprostone
- Calcium
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Topics |
- Animals
- Anti-Inflammatory Agents, Non-Steroidal
(administration & dosage, pharmacology, therapeutic use)
- Bupleurum
- Calcium
(metabolism)
- Cell Line, Tumor
(drug effects)
- Dinoprostone
(antagonists & inhibitors, biosynthesis)
- Dose-Response Relationship, Drug
- Glioma
(drug therapy, pathology)
- Oleanolic Acid
(administration & dosage, analogs & derivatives, pharmacology, therapeutic use)
- Phytotherapy
- Plant Roots
- Prostaglandin Antagonists
(administration & dosage, pharmacology, therapeutic use)
- Rats
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