Abstract | BACKGROUND: METHODS: Three groups (n = 10 pigs per group) had 75 minutes of left anterior descending artery occlusion during bypass. Group 1 had no treatment (control group), group 2 had controlled reperfusion (500 mL warm cardioplegia) (controlled reperfusion group), and group 3 had diazoxide (50 micromol/L before left anterior descending artery occlusion) ( diazoxide group). Biopsies were taken from the left anterior descending artery region before ischemia and at 3, 5, and 10 minutes postreperfusion. Intra-myocyte Na(+) and water contents were determined using atomic absorption spectroscopy, and Na(+) concentrations were calculated. RESULTS: Intra-myocyte Na(+) increased for the diazoxide group pigs at 3-minutes postreperfusion (21.9 +/- 2.9 vs 34.0 +/- 3.4 micromol/mL; p = 0.02), but decreased to 19.9 +/- 3.2 micromol/mL at 10 minutes postreperfusion (p = 1.0 vs baseline). At 10 minutes postreperfusion, intra-myocyte Na(+) in the controlled reperfusion group was lower than baseline (22.3 +/- 2.7 vs 17.2 +/- 3.1 micromol/mL; p < 0.001). Intra-myocyte Na(+) at 10 minutes postreperfusion for the diazoxide and controlled reperfusion groups was lower than for the control group (p < 0.05). CONCLUSIONS:
|
Authors | James E Davies, Stanley B Digerness, Steven P Goldberg, Cheryl R Killingsworth, Charles R Katholi, Paul S Brookes, William L Holman |
Journal | The Annals of thoracic surgery
(Ann Thorac Surg)
Vol. 76
Issue 4
Pg. 1252-8; discussion 1258
(Oct 2003)
ISSN: 0003-4975 [Print] Netherlands |
PMID | 14530020
(Publication Type: Comparative Study, Journal Article)
|
Chemical References |
|
Topics |
- Animals
- Diazoxide
(pharmacology)
- Female
- Homeostasis
(drug effects, physiology)
- Ischemic Preconditioning, Myocardial
(methods)
- Male
- Myocardial Reperfusion
(methods)
- Myocytes, Cardiac
(metabolism)
- Sodium
(metabolism)
- Swine
- Water-Electrolyte Balance
(drug effects, physiology)
|