Abstract | OBJECTIVE: METHODS: Cells were treated for 1 h and subsequently evaluated for apoptosis, survival, and caspase activity. Apoptosis was determined by fluorescent microscopy. Caspase-3, -8, and -9 activities were determined by fluorometry using target tetrapeptide substrates. Mitochondrial release of cytochrome c was determined by immunoblot analysis. RESULTS: CONCLUSIONS:
BMS 310705 induces significant apoptosis, decreases survival, and utilizes the mitochondrial-mediated pathway for apoptosis in this model.
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Authors | Denise Uyar, Nagio Takigawa, Tarek Mekhail, Dale Grabowski, Maurie Markman, Francis Lee, Renzo Canetta, Ron Peck, Ronald Bukowski, Ram Ganapathi |
Journal | Gynecologic oncology
(Gynecol Oncol)
Vol. 91
Issue 1
Pg. 173-8
(Oct 2003)
ISSN: 0090-8258 [Print] United States |
PMID | 14529678
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- BMS 310705
- Cytochrome c Group
- Epothilones
- Isoenzymes
- Organoplatinum Compounds
- Irinotecan
- Topotecan
- Caspases
- Paclitaxel
- Camptothecin
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Topics |
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Camptothecin
(analogs & derivatives, pharmacology)
- Caspases
(metabolism)
- Cytochrome c Group
(metabolism)
- Drug Resistance, Neoplasm
- Enzyme Activation
(drug effects)
- Epothilones
(pharmacology)
- Female
- Humans
- Irinotecan
- Isoenzymes
(metabolism)
- Organoplatinum Compounds
(pharmacology)
- Ovarian Neoplasms
(drug therapy, enzymology, pathology)
- Paclitaxel
(pharmacology)
- Topotecan
(pharmacology)
- Tumor Cells, Cultured
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