The human pituitary proteome: the characterization of differentially expressed proteins in an adenoma compared to a control.

Abstract

In order to clarify the basic molecular mechanisms that participate in the formation of human pituitary macroadenomas, this study, for the first time, describes the comparative proteomics between a pituitary adenoma tissue and a control tissue. A vertical, two-dimensional polyacrylamide gel electrophoresis system and PDQuest image analysis software were used to provide a high level of between-gel reproducibility and electrophoretic separation to accurately locate each differentially expressed protein. Mass spectrometry (MALDI-TOF and LC-ESI-Q-IT) and protein databases were used to characterize each differentially expressed protein. A total of 137 differential gel spots (37 increased spot volumes, 39 decreased, 19 new and 42 lost) were found when we compared an adenoma proteome to a control proteome. Seventy-one spots (20 increased, 27 decreased, 13 new, 11 lost), representing 39 differentially regulated proteins, were identified. Five differentially regulated proteins (prolactin, cellular retinoic acid-binding protein II, G-protein beta subunit 3, secretagogin and calreticulin) were also validated with results from a comparative transcriptomics study of pituitary adenomas and controls. The functional characteristics of these differentially expressed proteins provide a differential proteomic profile between a pituitary adenoma and a control.

• Authors: D M Desiderio, X Zhan
• Journal: Cellular and molecular biology (Noisy-le-Grand, France) (Cell Mol Biol (Noisy-le-grand)) Vol. 49 Issue 5 Pg. 689-712 (Jul 2003) ISSN: 0145-5680 [Print] France
• PMID: 14528906 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Proteome
• Trypsin

Topics

• Adenoma (genetics, metabolism)
• Amino Acid Sequence
• Analysis of Variance
• Chromatography, Liquid (methods)
• Computational Biology (methods)
• Databases, Protein
• Electronic Data Processing (methods)
• Electrophoresis, Gel, Two-Dimensional (methods)
• Gene Expression Regulation, Neoplastic
• Humans
• Hydrolysis
• Image Processing, Computer-Assisted (methods)
• Isoelectric Focusing (methods)
• Isoelectric Point
• Male
• Molecular Sequence Data
• Molecular Weight
• Peptide Mapping
• Pituitary Gland (chemistry, pathology)
• Pituitary Neoplasms (genetics, metabolism)
• Proteome (analysis, isolation & purification)
• Reproducibility of Results
• Silver Staining
• Spectrometry, Mass, Electrospray Ionization (methods)
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods)
• Trypsin (metabolism)