The effects of glucose-insulin-potassium solution and BN 52021 in intestinal ischemia-reperfusion injury.

Abstract

The objective of this study was to investigate effects of glucose-insulin-potassium (GIK) solution and BN 52021, a platelet-activating factor antagonist, on intestinal ischemia-reperfusion injury. Fifty male Sprague-Dawley rats (200-225 g) were divided into 5 groups each containing 10 rats; group SO, sham operation group; group I, mesenteric ischemia group (for 30 minutes); group R, ischemia plus reperfusion (for 60 minutes); group BR, ischemia-reperfusion plus BN 52021; group GR, ischemia-reperfusion plus GIK solution. Samples for malondialdehyde (MDA) and ileum (for mucosal injury score) were obtained. The mucosal injury scores of group R were significantly higher than those of group I (4 +/-0.20 and 3 +/-0.16, respectively, p<0.0001). The scores of groups BR and GR were significantly lower than those of group R (p<0.0001 and p<0.0001, respectively). When it was compared with the injuries in BR and GR groups, similar results were obtained in both groups (p=0.190). Mean MDA levels of group R were significantly higher than those of group I, BR and GR (131.33 +/-3.99 nmol/g, 93.74 +/-3.22 nmol/g, 104.81 +/-2.56 and 100.34 +/-5.30, respectively, p<0.0001). MDA levels of group BR and GR were significantly lower than those of group I (p<0.0001 and p=0.003, respectively). These observations suggest that treatment with GIK solution and BN 52021 before reperfusion and during reperfusion period may be useful in decreasing intestinal reperfusion injury.

• Authors: Mustafa Aldemir, Ahmet Gürel, Hüseyin Büyükbayram, Ibrahim Taçyildiz
• Journal: Vascular and endovascular surgery (Vasc Endovascular Surg) 2003 Sep-Oct Vol. 37 Issue 5 Pg. 345-51 ISSN: 1538-5744 [Print] United States
• PMID: 14528380 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Diterpenes
• Ginkgolides
• Insulin
• Lactones
• glucose-insulin-potassium cardioplegic solution
• ginkgolide B
• Glucose
• Potassium

Topics

• Animals
• Disease Models, Animal
• Diterpenes (pharmacology)
• Ginkgolides
• Glucose (pharmacology)
• Injury Severity Score
• Insulin (pharmacology)
• Intestinal Diseases (therapy)
• Lactones (pharmacology)
• Male
• Potassium (pharmacology)
• Probability
• Rats
• Rats, Sprague-Dawley
• Reference Values
• Reperfusion Injury (mortality, therapy)
• Risk Assessment
• Sensitivity and Specificity
• Survival Rate
• Treatment Outcome