[Relationship between insulin resistance and oxidative stress in vivo].

Abstract

OBJECTIVES:

The relationship between oxidative stress in vivo and insulin resistance was examined.

METHODS:

This study included 87 patients, 46 males and 41 females (mean age 63 +/- 10 years), without coronary artery disease. The homeostasis assessment insulin resistance (HOMA-IR) (fasting blood sugar x fasting immunoreactive insulin/405), a marker for insulin resistance, was measured. The patients were divided into three groups: the noninsulin resistance group (N-IR group) without diabetes mellitus (DM) and with fasting blood glucose level of 126 mg/dl and HOMA-IR < or = 1.73 (n = 44), the insulin resistance group (IR group) without diabetes mellitus and with fasting blood glucose level of 126 mg/dl and HOMA-IR > 1.73 (n = 29), and the DM group (type 2 diabetes mellitus) (n = 14). Urinary 8-iso-prostaglandin F2 alpha (U-8-iso-PGF2 alpha) excretion was measured as a marker of in vivo oxidative stress.

RESULTS:

There were significantly more obese patients in the IR group than in the N-IR group (62% vs 25%, p = 0.001), and the remnant-like particle cholesterol level was significantly higher in the IR group than in the N-IR group (7.6 +/- 5.2 vs 4.6 +/- 1.5 mg/dl, p < 0.01). Patients in the IR group had a significantly larger number of coronary risk factors. U-8-iso-PGF2 alpha excretion was significantly higher in the IR group and DM groups (201 +/- 86, 191 +/- 136 vs 129 +/- 50 pg/mg. Cr, p < 0.0001, p = 0.01), and there was a significantly positive correlation between the number of coronary risk factors, fasting blood sugar and U-8-iso-PGF2 alpha concentration (correlation coefficient = 0.32, 0.37, p = 0.002, p = 0.0003). Multiple regression analysis showed that remnant-like particle cholesterol, fasting blood sugar and insulin resistance were independent factors for U-8-iso-PGF2 alpha concentration (p < 0.0001, p = 0.0007, p = 0.02).

CONCLUSIONS:

Insulin resistance, remnant lipoprotein and hyperglyceridemia are deeply involved in oxidative stress in vivo.

• Authors: Takashi Hitsumoto, Takuo Iizuka, Mao Takahashi, Kunio Yoshinaga, Jun Matsumoto, Kazuhiro Shimizu, Michihisa Kaku, Yuko Sugiyama, Takeshi Sakurai, Kaneyuki Aoyagi, Masahito Kanai, Hirofumi Noike, Hidefumi Ohsawa, Hitoshi Watanabe, Kohji Shirai
• Journal: Journal of cardiology (J Cardiol) Vol. 42 Issue 3 Pg. 119-27 (Sep 2003) ISSN: 0914-5087 [Print] Netherlands
• PMID: 14526661 (Publication Type: English Abstract, Journal Article)

Chemical References

• Blood Glucose
• F2-Isoprostanes
• Lipoproteins
• 8-epi-prostaglandin F2alpha
• Cholesterol
• Dinoprost

Topics

• Blood Glucose (analysis)
• Cholesterol (blood)
• Diabetes Mellitus (blood)
• Dinoprost (analogs & derivatives)
• F2-Isoprostanes (urine)
• Female
• Homeostasis (physiology)
• Humans
• Insulin Resistance (physiology)
• Lipoproteins (blood)
• Male
• Middle Aged
• Oxidative Stress (physiology)
• Regression Analysis