Identification of proNeuropeptide FFA peptides processed in neuronal and non-neuronal cells and in nervous tissue.

Abstract

Peptides which should be generated from the neuropeptide FF (NPFF) precursor were identified in a neuronal (human neuroblastoma SH-SY5Y) cell line and in COS-7 cells after transient transfection of the human proNPFFA cDNA and were compared with those detected in the mouse spinal cord. After reverse-phase high performance liquid chromatography of soluble material, NPFF-related peptides were immunodetected with antisera raised against NPFF and identified by using on-line capillary liquid chromatography/nanospray ion trap tandem mass spectrometry. Neuronal and non-neuronal cells generated different peptides from the same precursor. In addition to NPFF, SQA-NPFF (Ser-Gln-Ala-Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-amide) and NPAF were identified in the human neuroblastoma while only NPFF was clearly identified in COS-7 cells. In mouse, in addition to previously detected NPFF and NPSF, SPA-NPFF (Ser-Pro-Ala-Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-amide), the homologous peptide of SQA-NPFF, were characterized. These data on intracellular processing of proNeuropeptide FFA are discussed in regard to the known enzymatic processing mechanisms.

• Authors: Elisabeth Bonnard, Odile Burlet-Schiltz, Bernard Monsarrat, Jean-Philippe Girard, Jean-Marie Zajac
• Journal: European journal of biochemistry (Eur J Biochem) Vol. 270 Issue 20 Pg. 4187-99 (Oct 2003) ISSN: 0014-2956 [Print] England
• PMID: 14519131 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Oligopeptides
• Peptides
• phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide

Topics

• Animals
• COS Cells
• Chromatography, High Pressure Liquid
• Humans
• Mass Spectrometry
• Mice
• Neuroblastoma (metabolism)
• Oligopeptides (isolation & purification)
• Peptides (isolation & purification)
• Spinal Cord (metabolism)