Abstract |
One- and two-dimensional gel electrophoretic analysis of nuclear proteins of Kirkman-Robbins hepatoma was used to study the effects of the tumour growth inhibitors methotrexate (MTX) and acyclonucleoside ( DHPQtB) on protein composition. MTX and DHPQtB inhibited Kirkman-Robbins hepatoma growth by 89.2 +/- 3.5% and 16.3 +/- 6.1% respectively. The biosynthesis and/or metabolism of some polypeptide spots was affected by these antitumour agents, especially among components with molecular wt/isoelectric points of 52,000-64,000/4.9-5.5, 69,000-78,000/5.0-5.9 and 88,000-100,000/5.1-5.9.
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Authors | Z Kiliańska, A Lipińska, W M Krajewska, M Gaczyński, J Bartkowiak, L Kłyszejko-Stefanowicz |
Journal | Cytobios
(Cytobios)
Vol. 70
Issue 281
Pg. 91-100
( 1992)
ISSN: 0011-4529 [Print] England |
PMID | 1451536
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antimetabolites, Antineoplastic
- Neoplasm Proteins
- Nuclear Proteins
- Quinazolines
- 1-N-(1,3-dihydroxy-2-propoxymethyl)-6-(tert-butyl)-5,6,7,8-tetrahydro-2,4-quinazolinedione
- Testosterone
- Methotrexate
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Topics |
- Animals
- Antimetabolites, Antineoplastic
(pharmacology, therapeutic use)
- Cricetinae
- Depression, Chemical
- Electrophoresis, Gel, Two-Dimensional
- Electrophoresis, Polyacrylamide Gel
- Gene Expression Regulation, Neoplastic
(drug effects)
- Liver Neoplasms, Experimental
(chemistry, drug therapy)
- Male
- Mesocricetus
- Methotrexate
(pharmacology, therapeutic use)
- Molecular Weight
- Neoplasm Proteins
(isolation & purification, metabolism)
- Neoplasms, Hormone-Dependent
(chemistry, drug therapy)
- Nuclear Proteins
(isolation & purification, metabolism)
- Quinazolines
(pharmacology, therapeutic use)
- Testosterone
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