Abstract | BACKGROUND: METHODS: Experiments were performed at 37 degrees C on continuously perfused monolayers of human neuroblastoma SH-SY5Y cells using Fura-2 as the cytoplasmic Ca2+ indicator. Carbachol (1 mm) was applied to evoke a transient increase in cytoplasmic Ca2+. RESULTS:
Isoflurane (1 mm) reduces the carbachol-evoked transient increase in cytoplasmic Ca2+, and this isoflurane action is eliminated when the cells are continuously stimulated with 200 mm KCl or pretreated with 10 mm caffeine or 200 microm ryanodine. CONCLUSIONS:
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Authors | Alexandra Corrales, Fang Xu, Zayra Garavito-Aguilar, Thomas J J Blanck, Esperanza Recio-Pinto |
Journal | Anesthesiology
(Anesthesiology)
Vol. 99
Issue 4
Pg. 882-8
(Oct 2003)
ISSN: 0003-3022 [Print] United States |
PMID | 14508321
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Calcium Channels
- Caffeine
- Carbachol
- Isoflurane
- Calcium
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Topics |
- Caffeine
(pharmacology)
- Calcium
(metabolism)
- Calcium Channels
(metabolism)
- Carbachol
(antagonists & inhibitors, pharmacology)
- Cytoplasm
(drug effects, physiology)
- Drug Interactions
(physiology)
- Humans
- Isoflurane
(pharmacology)
- Tumor Cells, Cultured
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