Abstract | BACKGROUND: STUDY DESIGN AND METHODS: PLT-reactive antibodies were characterized by three different solid-phase assays and by flow cytometry. RESULTS: The two maternal antibodies gave negative reactions in the antigen capture ELISA, modified antigen capture ELISA, and MoAb immobilization of PLT antigens tests but reacted strongly in flow cytometry with intact PLTs that were HPA-3a+. Other sera samples specific for HPA-3a reacted equally well in all assays. CONCLUSIONS: The two antibodies appear to recognize an epitope on the HPA-3a+ form of glycoprotein IIb that is lost when PLTs are solubilized in detergent, as required for solid-phase assays. The diagnosis was made in these cases because no HLA antibodies were present, allowing an HPA-3a-specific reaction to be identified with intact PLTs as targets. Such antibodies are likely to be overlooked when HLA antibodies are also present.
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Authors | C R Harrison, B R Curtis, J G McFarland, R W Huff, R H Aster |
Journal | Transfusion
(Transfusion)
Vol. 43
Issue 10
Pg. 1398-402
(Oct 2003)
ISSN: 0041-1132 [Print] United States |
PMID | 14507271
(Publication Type: Case Reports, Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- 3a alloantigen, human
- Antigens, Human Platelet
- Epitopes
- Isoantibodies
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Topics |
- Adult
- Antigens, Human Platelet
(immunology)
- Blood Platelets
(immunology)
- Epitopes
- Female
- Flow Cytometry
- Humans
- Infant, Newborn
- Isoantibodies
(blood, immunology)
- Thrombocytopenia
(etiology, immunology)
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