Evaluation of the roles of four Candida albicans genes in virulence by using gene disruption strains that express URA3 from the native locus.

Abstract

Reintroducing URA3 to its native locus in Candida albicans not5, not3, bur2, and kel1 disruption mutants enabled us to directly compare strains with control strain CAI-12. We showed that URA3 position affected orotidine 5'-monophosphate decarboxylase activity, hyphal morphogenesis, adherence, and mortality in murine disseminated candidiasis. After URA3 was reintroduced to its native locus, only NOT5 could be conclusively ascribed a role in virulence.

• Authors: Shaoji Cheng, M Hong Nguyen, Zongde Zhang, Hongyan Jia, Martin Handfield, Cornelius J Clancy
• Journal: Infection and immunity (Infect Immun) Vol. 71 Issue 10 Pg. 6101-3 (Oct 2003) ISSN: 0019-9567 [Print] United States
• PMID: 14500538 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Fungal Proteins

Topics

• Animals
• Candida albicans (genetics, pathogenicity)
• Candidiasis (microbiology)
• Cell Adhesion
• Fungal Proteins (genetics)
• Genes, Fungal
• Humans
• In Vitro Techniques
• Mice
• Mutation
• Phenotype
• Virulence (genetics)