Effect of three triterpenoid compounds isolated from root bark of Aralia elata on stimulus-induced superoxide generation and tyrosyl phosphorylation and translocation of p47(phox) and p67(phox) to cell membrane in human neutrophil.

Abstract

BACKGROUND:

Root bark of Aralia elata is used as a folk medicine for neurasthenia, rheumatism, diabetes, hepatitis virus and spasm of the stomach in China, Japan and Russia.

METHODS:

The effect of three triterpenoid compounds isolated from root bark of A. elata on stimulus-induced superoxide generation and tyrosyl phosphorylation and translocation of p47(phox) and p67(phox) to cell membrane was investigated. The three compounds examined were Elatoside A, Elatoside C, and Tarasaponin V.

RESULTS:

When the cells were preincubated with these compounds, the superoxide generation induced by N-formyl-methionyl-leucyl-phenylalanine (fMLP) was suppressed in a low concentration range. However, the superoxide generation was significantly enhanced by 40 micromol/l triterpenoid, and was again suppressed in the higher concentration range. In the case of superoxide generation induced by phorbol 12-myristate 13-acetate (PMA), the compounds had no obvious effect on the superoxide generation in low concentration but suppressed that at 40 micromol/l. These compounds also efficiently suppressed the superoxide generation induced by arachidonic acid (AA) at 10 micromol/l. In parallel to the effect on the fMLP-induced superoxide generation, these compounds suppressed fMLP-induced tyrosyl phosphorylation and the translocation to membrane of cytosolic compounds, p47(phox) and p67(phox) at 10 and 80 micromol/l but not at 40 micromol/l.

CONCLUSIONS:

Triterpenoid saponins examined in this study effect stimulus-induced superoxide generation and tyrosyl phosphorylation and translocation to membrane of p47(phox) and p67(phox) in a concentration-dependent manner, and may have some pharmaceutical applications.

• Authors: Zhigang Wang, Shaojiang Song, Huangwei Lu, Guang Chen, Suixu Xu, Yasuhiro Sagara, Noriko Kitaoka, Masanobu Manabe, Hiroyuki Kodama
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 336 Issue 1-2 Pg. 65-72 (Oct 2003) ISSN: 0009-8981 [Print] Netherlands
• PMID: 14500036 (Publication Type: Journal Article)

Chemical References

• Phosphoproteins
• Saponins
• Triterpenes
• neutrophil cytosol factor 67K
• Superoxides
• Arachidonic Acid
• Tyrosine
• phorbolol myristate acetate
• N-Formylmethionine Leucyl-Phenylalanine
• NADPH Oxidases
• neutrophil cytosolic factor 1
• Tetradecanoylphorbol Acetate

Topics

• Arachidonic Acid (pharmacology)
• Aralia (chemistry)
• Cell Membrane (metabolism)
• Humans
• Immunoblotting
• Molecular Structure
• N-Formylmethionine Leucyl-Phenylalanine (pharmacology)
• NADPH Oxidases
• Neutrophils (drug effects, metabolism)
• Phosphoproteins (blood)
• Phosphorylation
• Plant Bark (chemistry)
• Plant Roots (chemistry)
• Protein Transport (drug effects)
• Saponins (chemistry, pharmacology)
• Superoxides (blood)
• Tetradecanoylphorbol Acetate (analogs & derivatives, pharmacology)
• Triterpenes (chemistry, pharmacology)
• Tyrosine (blood)