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Rhizobium meliloti elicits transient expression of the early nodulin gene ENOD12 in the differentiating root epidermis of transgenic alfalfa.

Abstract
To study the molecular responses of the host legume during early stages of the symbiotic interaction with Rhizobium, we have cloned and characterized the infection-related early nodulin gene MtENOD12 from Medicago truncatula. In situ hybridization experiments have shown that, within the indeterminate Medicago nodule, transcription of the MtENOD12 gene begins in cell layers of meristematic origin that lie ahead of the infection zone, suggesting that these cells are undergoing preparation for bacterial infection. Histochemical analysis of transgenic alfalfa plants that express an MtENOD12 promoter-beta-glucuronidase gene fusion has confirmed this result and further revealed that MtENOD12 gene transcription occurs as early as 3 to 6 hr following inoculation with R. meliloti in a zone of differentiating root epidermal cells which lies close to the growing root tip. It is likely that this transient, nodulation (nod) gene-dependent activation of the ENOD12 gene also corresponds to the preparation of the plant for bacterial infection. We anticipate that this extremely precocious response to Rhizobium will provide a valuable molecular marker for studying early signal exchange between the two symbiotic organisms.
AuthorsM Pichon, E P Journet, A Dedieu, F de Billy, G Truchet, D G Barker
JournalThe Plant cell (Plant Cell) Vol. 4 Issue 10 Pg. 1199-211 (Oct 1992) ISSN: 1040-4651 [Print] England
PMID1446169 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • MTN6 protein, Medicago truncatula
  • Membrane Proteins
  • Plant Proteins
  • nodulin
  • Glucuronidase
Topics
  • Amino Acid Sequence
  • Base Sequence
  • Cell Differentiation (genetics)
  • Cloning, Molecular
  • Gene Expression Regulation
  • Genes, Plant
  • Glucuronidase
  • Medicago sativa (genetics, growth & development)
  • Membrane Proteins
  • Molecular Sequence Data
  • Nitrogen Fixation (genetics)
  • Plant Proteins (genetics, isolation & purification)
  • Plants, Genetically Modified (genetics, growth & development)
  • Promoter Regions, Genetic
  • Sequence Homology, Nucleic Acid
  • Sinorhizobium meliloti (genetics, physiology)
  • Symbiosis
  • Transcription, Genetic

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