In vitro studies on the influence of L-ascorbic acid 2-[3,4-dihydro- 2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-1-benzopyran-6yl-hy drogen phosphate] potassium salt on lipid peroxidation and phospholipase A2 activity.

Abstract

The effects of L-ascorbic acid 2-[3,4-dihydro-2,5,7,8-tetramethyl-2- (4,8,12-trimethyltridecyl)-2H-1-benzopyran-6yl-hydrogen phosphate] potassium salt (EPC-K1, CAS 127061-56-7), a new compound for ischemia-reperfusion injuries, on lipid peroxidation and phospholipase A2 activity were studied in vitro using rat brain homogenates and human plasma. EPC-K1 inhibited phospholipase A2 activity in human plasma in a concentration-dependent manner (IC50 = 7.3 x 10(-4) mol/l), whereas a mixture of alpha-tocopherol and ascorbic acid did not exhibit this effect. In rat brain homogenates, EPC-K1 also inhibited lipid peroxidation in a concentration-dependent manner (IC50 = 2.3 x 10(-6) mol/l). alpha-Tocopherol was less active than EPC-K1. These properties of EPC-K1 suggest that EPC-K1 may prove useful in the treatment of ischemia-reperfusion injuries.

• Authors: Y Kuribayashi, K Yoshida, T Sakaue, A Okumura
• Journal: Arzneimittel-Forschung (Arzneimittelforschung) Vol. 42 Issue 9 Pg. 1072-4 (Sep 1992) ISSN: 0004-4172 [Print] Germany
• PMID: 1445471 (Publication Type: Journal Article)

Chemical References

• Vitamin E
• Phospholipases A
• Phospholipases A2
• Ascorbic Acid

Topics

• Animals
• Ascorbic Acid (pharmacology)
• Brain (enzymology)
• Brain Chemistry (drug effects)
• In Vitro Techniques
• Lipid Peroxidation (drug effects)
• Male
• Phospholipases A (antagonists & inhibitors)
• Phospholipases A2
• Rats
• Rats, Wistar
• Vitamin E (pharmacology)