Experimental colitis in mice: effects of olsalazine on eicosanoid production in colonic tissue.

Abstract

In rodents colitis can be induced by adding 2% (w/v) carrageenan (CARR) for 4 weeks or 10% (w/v) dextran sulphate sodium (DSS) for 7 days to their drinking water. These models are suitable to test anti-inflammatory drugs used in inflammatory bowel disease in man. Mice were treated with olsalazine (400 mg/kg body wt) starting 7 days before the DSS or CARR administration. Colonic tissues were incubated with [1-14C]-arachidonic acid and stimulated with A23187 and, thereafter, the pattern of eicosanoids was determined by separation on HPLC. DSS and CARR produced a marked diffuse inflammatory response in the colon and a subsequent 5-fold increase of all eicosanoids after DSS, whereas after CARR only a 2-fold increase of PGs was observed. Olsalazine treatment decreased all cyclooxygenase and lipoxygenase products to baseline levels.

• Authors: F J Zijlstra, I M Garrelds, A P van Dijk, J H Wilson
• Journal: Agents and actions (Agents Actions) Vol. Spec No Pg. C76-8 ( 1992) ISSN: 0065-4299 [Print] Switzerland
• PMID: 1442339 (Publication Type: Journal Article)

Chemical References

• Aminosalicylic Acids
• Eicosanoids
• Arachidonic Acid
• Calcimycin
• Carrageenan
• Dextran Sulfate
• olsalazine

Topics

• Aminosalicylic Acids (pharmacology)
• Animals
• Arachidonic Acid (pharmacology)
• Calcimycin (pharmacology)
• Carrageenan
• Colitis (chemically induced, drug therapy, metabolism)
• Colon (drug effects, metabolism)
• Dextran Sulfate
• Disease Models, Animal
• Eicosanoids (biosynthesis)
• Female
• Intestinal Mucosa (drug effects, metabolism)
• Mice
• Mice, Inbred BALB C