Abstract |
The antitumor activity of GP-7, a new spin-labeled epipodophyllotoxin, was studied by liquid scintillation spectrometry. There were many similarities between GP-7 and etoposide. Both GP-7 and etoposide inhibited the incorporation of [3H]TdR, [3H]UR, and [3H]Leu into DNA, RNA, and protein synthesis in leukemia 7712 cells. The inhibition correlated with drug concentration and duration. IC50 of GP-7 and etoposide on DNA synthesis at 24 h were 0.21 and 0.37 micrograms.ml-1, respectively. The inhibition of GP-7 or etoposide on DNA synthesis retained even after the drug were washed out for 3 h. GP-7 and etoposide caused DNA single-strand breaks, with a well concentration-response relationship. These data suggest that the inhibition of DNA synthesis by GP-7 or etoposide is likely due to the damage of DNA template and breaking of single-strand DNA.
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Authors | X Q He, P Y Zhang, X Tian, J X Li |
Journal | Zhongguo yao li xue bao = Acta pharmacologica Sinica
(Zhongguo Yao Li Xue Bao)
Vol. 13
Issue 3
Pg. 276-9
(May 1992)
ISSN: 0253-9756 [Print] China |
PMID | 1442115
(Publication Type: English Abstract, Journal Article)
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Chemical References |
- Antineoplastic Agents, Phytogenic
- DNA, Neoplasm
- RNA, Neoplasm
- GP 7
- Etoposide
- Podophyllotoxin
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Topics |
- Animals
- Antineoplastic Agents, Phytogenic
(pharmacology)
- DNA Damage
(drug effects)
- DNA, Neoplasm
(biosynthesis)
- Etoposide
(pharmacology)
- Leukemia, Experimental
(pathology)
- Mice
- Podophyllotoxin
(analogs & derivatives, pharmacology)
- RNA, Neoplasm
(biosynthesis)
- Tumor Cells, Cultured
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