[The use of an immunohistochemical method for detecting N7-methylguanine in human cells].

Abstract

An immunohistochemical method, followed by silvering (Ag-treatment) of the final product of peroxidase reaction for N7-methylguanine detection, was first employed for human cells treated in vitro with N-methylnitrosourea. This method appeared to be highly specific and sensitive, compared to the routine immunohistochemical technique. Evidence has been provided on the application of Ag-treatment for detecting N7-methylguanine in the esophagus epithelium of patients with a high risk of cancer with this particular locality.

• Authors: A M Mikheev
• Journal: Tsitologiia (Tsitologiia) Vol. 34 Issue 3 Pg. 57-61 ( 1992) ISSN: 0041-3771 [Print] Russia (Federation)
• Vernacular Title: Ispol'zovanie immunogistokhimicheskogo metoda dlia vyiavleniia N7-metilguanina v kletkakh cheloveka.
• PMID: 1440930 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Guanine
• 7-methylguanine
• Methylnitrosourea
• DNA

Topics

• China
• DNA (drug effects, metabolism)
• Disease Reservoirs
• Epithelial Cells
• Epithelium (drug effects, metabolism)
• Esophageal Neoplasms (metabolism, pathology)
• Esophagus (cytology, drug effects, metabolism)
• Guanine (analogs & derivatives, metabolism)
• Humans
• Immunohistochemistry (methods)
• Intestinal Mucosa (metabolism)
• Intestines (cytology, drug effects)
• Methylnitrosourea (pharmacology)
• Sensitivity and Specificity
• Silver Staining (methods)