FRACTIONATION OF THYMIDINE PHOSPHOKINASE, THYMIDINE 5'-MONOPHOSPHATE PHOSPHOKINASE AND THYMIDINE 5'-DIPHOSPHATE PHOSPHOKINASE IN EXTRACTS OF LANDSCHUTZ ASCITES-TUMOUR CELLS.

Abstract

1. Extracts of Landschutz ascites-tumour cells have been fractionated by treatment with acid, alumina C(gamma) gel and Sephadex G-100 to yield purified preparations of thymidine phosphokinase, thymidine 5'-monophosphate phosphokinase and thymidine 5'-diphosphate phosphokinase. 2. These results clearly demonstrate the existence in Landschutz ascites tumour of three phosphokinases each of which catalyses one step in the reaction sequence: thymidineright harpoon over left harpoonthymidine 5'-monophosphateright harpoon over left harpoonthymidine 5'-diphosphateright harpoon over left harpoonthymidine 5'-triphosphate. Though these results do not preclude the participation of other enzymes in the formation of thymidine 5'-triphosphate from thymidine by Landschutz ascites-tumour cells, they provide strong support for the view that thymidine 5'-diphosphate is an intermediate in the formation of thymidine 5'-triphosphate from thymidine 5'-monophosphate by this system.

• Authors: H J GRAV, R M SMELLIE
• Journal: The Biochemical journal (Biochem J) Vol. 94 Pg. 518-24 (Mar 1965) ISSN: 0264-6021 [Print] England
• PMID: 14340042 (Publication Type: Journal Article)

Chemical References

• Dextrans
• Nucleotides
• Thymine Nucleotides
• thymidine 5'-diphosphate
• sephadex
• Phosphotransferases
• Thymidine Kinase
• Nucleoside-Phosphate Kinase
• dTMP kinase
• thymidine 5'-triphosphate
• Thymidine

Topics

• Ascites
• Biochemical Phenomena
• Biochemistry
• Chemistry Techniques, Analytical
• Chromatography
• Chromatography, Gel
• Dextrans
• Neoplasms
• Nucleoside-Phosphate Kinase
• Nucleotides
• Phosphotransferases
• Research
• Spectrophotometry
• Thymidine
• Thymidine Kinase
• Thymine Nucleotides
• Tissue Culture Techniques