Post-transcriptional regulation by interferon-alpha of epsilon-globin production in human erythroleukemia K-562 cells.

Abstract

Treatment of human K-562 erythroleukemia cells with interferon-alpha (IFN-alpha) resulted in a six-fold increase in the production of epsilon-globin, a component of embryonic hemoglobin, but in a two-fold or less augmentation of the production of other globin species. There was no comparable increase in the production of epsilon-globin mRNA following IFN treatment. We did, however, observe a shift in the cytoplasmic location of the epsilon-globin mRNA from the messenger ribonucleoprotein (mRNP) to the polysome fraction after IFN-alpha addition. No such shift was noted in the location of the mRNA for A gamma-globin, the major globin product of K-562 cells, which was always present in the polysome fraction. These results are significant with respect to the mechanisms of cellular regulation and differentiation induced by IFNs.

• Authors: R M Friedman, A Yeh, W Tang
• Journal: Journal of interferon research (J Interferon Res) Vol. 12 Issue 4 Pg. 311-6 (Aug 1992) ISSN: 0197-8357 [Print] United States
• PMID: 1431310 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• DNA Probes
• DNA, Neoplasm
• Hemoglobins
• Interferon-alpha
• RNA, Messenger
• RNA, Neoplasm
• Ribonucleoproteins
• Globins

Topics

• Antibodies, Monoclonal
• Cytosol (metabolism)
• DNA Probes
• DNA, Neoplasm (analysis)
• Globins (biosynthesis, genetics)
• Hemoglobins (analysis)
• Humans
• Interferon-alpha (pharmacology)
• Leukemia, Erythroblastic, Acute (metabolism)
• Polyribosomes (metabolism)
• RNA, Messenger (biosynthesis)
• RNA, Neoplasm (analysis)
• Ribonucleoproteins (metabolism)
• Transcription, Genetic (genetics)
• Tumor Cells, Cultured