HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Mass spectrometric analysis of the HMGY protein from Lewis lung carcinoma. Identification of phosphorylation sites.

Abstract
The primary structure of the Lewis lung carcinoma protein HMGY belonging to the nuclear group of proteins HMGI (high mobility group I) was determined using electrospray and fast atom bombardment mass spectrometry. It was demonstrated that the sequence of the tumor protein corresponds to the amino acid sequence derived from the cDNA from cultured cells and that the N-terminal serine residue is N-acetylated. Moreover, the two high performance liquid chromatography-purified forms Y1 and Y2 of the protein HMGY were shown to differ at the level of serine phosphorylation, since they contain three phosphate and two phosphate groups, respectively, in the C-terminal region. No other modification was detected in the remaining part of the molecule.
AuthorsP Ferranti, A Malorni, G Marino, P Pucci, G H Goodwin, G Manfioletti, V Giancotti
JournalThe Journal of biological chemistry (J Biol Chem) Vol. 267 Issue 31 Pg. 22486-9 (Nov 05 1992) ISSN: 0021-9258 [Print] United States
PMID1429598 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • High Mobility Group Proteins
  • Neoplasm Proteins
  • Peptide Fragments
  • Phosphoproteins
  • DNA
Topics
  • Amino Acid Sequence
  • Animals
  • DNA (genetics)
  • High Mobility Group Proteins (chemistry)
  • Lung Neoplasms (chemistry)
  • Mass Spectrometry
  • Mice
  • Molecular Sequence Data
  • Neoplasm Proteins (chemistry)
  • Peptide Fragments (chemistry)
  • Phosphoproteins (chemistry)
  • Phosphorylation

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: