Detection of Mycobacterium paratuberculosis antigen with colloidal immunogold in naturally infected sheep.

Abstract

The anti-Mycobacterium paratuberculosis polyclonal serum is proved useful for labelling Mycobacterium paratuberculosis in glutaraldehyde-osmium-fixed and epon-embedded intestinal samples from sheep with clinical symptoms of paratuberculosis. M. paratuberculosis marked with antibody-coated colloidal gold stain was seen in macrophages, epithelioid cells, giant cells and neutrophils throughout intestinal mucosa. In large macrophages with a low lysosomal content, a great number of intact mycobacteria was seen within phagosomes. In macrophages with average lysosomal content, very few intact mycobacteria or mycobacterial debris were present and lysosome-phagosome fusions were observed. Mycobacteria within neutrophils were scanty. These results show the usefulness of colloidal immunogold techniques for studies of the pathogenesis of paratuberculosis.

• Authors: J A Navarro, J Sanchez, A Bernabe, M A Gomez, S Gomez, J Seva
• Journal: Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B (Zentralbl Veterinarmed B) Vol. 39 Issue 6 Pg. 421-7 (Aug 1992) ISSN: 0514-7166 [Print] Germany
• PMID: 1414096 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Bacterial

Topics

• Animals
• Antigens, Bacterial (analysis)
• Immunohistochemistry
• Microscopy, Immunoelectron
• Mycobacterium avium subsp. paratuberculosis (immunology)
• Paratuberculosis (microbiology)
• Phagocytes (microbiology)
• Sheep
• Sheep Diseases (microbiology)