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Identification of the CTAG-recognizing restriction-modification systems MthZI and MthFI from Methanobacterium thermoformicicum and characterization of the plasmid-encoded mthZIM gene.

Abstract
Two CTAG-recognizing restriction and modification (R/M) systems, designated MthZI and MthFI, were identified in the thermophilic archaeon Methanobacterium thermoformicicum strains Z-245 and FTF, respectively. Further analysis revealed that the methyltransferase (MTase) genes are plasmid-located in both strains. The plasmid pFZ1-encoded mthZIM gene of strain Z-245 was further characterized by subcloning and expression studies in Escherichia coli followed by nucleotide sequence analysis. The mthZIM gene is 1065 bp in size and may code for a protein of 355 amino acids (M(r) 42,476 Da). The deduced amino acid sequence of the M.MthZI enzyme shares substantial similarity with four distinct regions from several m4C- and m6A-MTases, and contains the TSPPY motif that is so far only found in m4C-MTases. Partially overlapping with the mthZIM gene and in reverse orientation, an additional ORF was identified with a size of 606 bp potentially coding for a protein of 202 amino acids (M(r) 23.710 Da). This ORF is suggested to encode the corresponding endonuclease R.MthZI.
AuthorsJ Nölling, W M de Vos
JournalNucleic acids research (Nucleic Acids Res) Vol. 20 Issue 19 Pg. 5047-52 (Oct 11 1992) ISSN: 0305-1048 [Print] England
PMID1408820 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • DNA, Bacterial
  • CTAG-specific type II deoxyribonucleases
  • Deoxyribonucleases, Type II Site-Specific
Topics
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial (genetics, isolation & purification)
  • Deoxyribonucleases, Type II Site-Specific (genetics)
  • Escherichia coli (genetics)
  • Genes, Bacterial
  • Methanobacterium (enzymology, genetics)
  • Molecular Sequence Data
  • Plasmids
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Substrate Specificity

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