Abstract |
Two CTAG-recognizing restriction and modification (R/M) systems, designated MthZI and MthFI, were identified in the thermophilic archaeon Methanobacterium thermoformicicum strains Z-245 and FTF, respectively. Further analysis revealed that the methyltransferase (MTase) genes are plasmid-located in both strains. The plasmid pFZ1-encoded mthZIM gene of strain Z-245 was further characterized by subcloning and expression studies in Escherichia coli followed by nucleotide sequence analysis. The mthZIM gene is 1065 bp in size and may code for a protein of 355 amino acids (M(r) 42,476 Da). The deduced amino acid sequence of the M.MthZI enzyme shares substantial similarity with four distinct regions from several m4C- and m6A-MTases, and contains the TSPPY motif that is so far only found in m4C-MTases. Partially overlapping with the mthZIM gene and in reverse orientation, an additional ORF was identified with a size of 606 bp potentially coding for a protein of 202 amino acids (M(r) 23.710 Da). This ORF is suggested to encode the corresponding endonuclease R.MthZI.
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Authors | J Nölling, W M de Vos |
Journal | Nucleic acids research
(Nucleic Acids Res)
Vol. 20
Issue 19
Pg. 5047-52
(Oct 11 1992)
ISSN: 0305-1048 [Print] England |
PMID | 1408820
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- DNA, Bacterial
- CTAG-specific type II deoxyribonucleases
- Deoxyribonucleases, Type II Site-Specific
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Topics |
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
(genetics, isolation & purification)
- Deoxyribonucleases, Type II Site-Specific
(genetics)
- Escherichia coli
(genetics)
- Genes, Bacterial
- Methanobacterium
(enzymology, genetics)
- Molecular Sequence Data
- Plasmids
- Restriction Mapping
- Sequence Homology, Amino Acid
- Substrate Specificity
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