Abstract |
A highly malignant human T-cell receptor (TCR) gamma/delta+ T-cell leukemia was shown to have a productive rearrangement of the TCR delta locus on one chromosome 14 and a novel t(8;14)(q24;q11) rearrangement involving the J delta 1 gene segment on the other chromosome 14. Chromosome walking coupled with pulsed-field gel electrophoretic (PFGE) analysis determined that the TCR J delta 1 gene fragment of the involved chromosome was relocated approximately 280 kb downstream of the c-myc proto-oncogene locus found on chromosome band 8q24. This rearrangement was reminiscent of the Burkitt's lymphoma variants that translocate to a region identified as the pvt-1 locus. Sequence comparison of the breakpoint junctions of interchromosomal rearrangements in T-cell leukemias involving the TCR delta-chain locus revealed novel signal-like sequence motifs, GCAGA(A/T)C and CCCA(C/G)GAC. These sequences were found on chromosome 8 at the 5' flanking site of the breakpoint junction of chromosome 8 in the TCR gamma/delta leukemic cells reported here and also on chromosome 1 in T-cell acute lymphocytic leukemia patients carrying the t(1;14)(p32;q11) rearrangement. These results suggest that (i) during early stages of gamma delta T-cell ontogeny, the region 280 kb 3' of the c-myc proto-oncogene on chromosome 8 is fragile and accessible to the lymphoid recombination machinery and (ii) rearrangements to both 8q24 and 1p32 may be governed by novel sequence motifs and be subject to common enzymatic mechanisms.
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Authors | M Kasai, R T Maziarz, K Aoki, E Macintyre, J L Strominger |
Journal | Molecular and cellular biology
(Mol Cell Biol)
Vol. 12
Issue 10
Pg. 4751-7
(Oct 1992)
ISSN: 0270-7306 [Print] United States |
PMID | 1406658
(Publication Type: Case Reports, Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- DNA, Neoplasm
- MAS1 protein, human
- Proto-Oncogene Mas
- Receptors, Antigen, T-Cell, gamma-delta
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Topics |
- Base Sequence
- Chromosomes, Human, Pair 14
- Chromosomes, Human, Pair 8
- DNA, Neoplasm
- Electrophoresis, Gel, Pulsed-Field
- Humans
- Leukemia, T-Cell
(genetics)
- Molecular Sequence Data
- Proto-Oncogene Mas
- Receptors, Antigen, T-Cell, gamma-delta
(genetics)
- Restriction Mapping
- Translocation, Genetic
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