[Cloning, primary structure determination and expression of preproinsulin cDNA from human insulinoma in Escherichia coli].

Abstract

A human insulinoma cDNA library was constructed in expression plasmid vector pUEX1. Clone pUEX1Ins12 was selected from human insulinoma cDNA library by means of hybridization with the insulin probe and a nucleotide sequence of the insertion was determined. It codes for full size amino acid sequence preproinsulin and furthermore, contains the entire 3'-end of noncoding mRNA region and 44 nucleotides from the 5'-untranslated region. The bacterial strain pUEX3Ins8 producing preproinsulin as beta-galactosidase fusion protein was constructed.

• Authors: M K Chekhranova, E R Shuvalova, A M Kutin, V Iu Butnev, A B Valentsova, E N Il'ina, Iu A Pankov
• Journal: Molekuliarnaia biologiia (Mol Biol (Mosk)) 1992 May-Jun Vol. 26 Issue 3 Pg. 596-600 ISSN: 0026-8984 [Print] Russia (Federation)
• Vernacular Title: Klorirovanie, opredelenie pervichnoĭ struktury i ékspressiia kDNK preproinsulina iz insulinomy cheloveka v Escherichia coli.
• PMID: 1406615 (Publication Type: Journal Article)

Chemical References

• Insulin
• Protein Precursors
• preproinsulin
• DNA
• Proinsulin

Topics

• Amino Acid Sequence
• Base Sequence
• Cloning, Molecular
• DNA
• Escherichia coli (genetics)
• Gene Expression
• Humans
• Insulin
• Insulinoma (metabolism)
• Molecular Sequence Data
• Plasmids
• Proinsulin (genetics)
• Protein Precursors (genetics)