A nonapeptide encoded by human gene MAGE-1 is recognized on HLA-A1 by cytolytic T lymphocytes directed against tumor antigen MZ2-E.

Abstract

We have reported the identification of human gene MAGE-1, which directs the expression of an antigen recognized on a melanoma by autologous cytolytic T lymphocytes (CTL). We show here that CTL directed against this antigen, which was named MZ2-E, recognize a nonapeptide encoded by the third exon of gene MAGE-1. The CTL also recognize this peptide when it is presented by mouse cells transfected with an HLA-A1 gene, confirming the association of antigen MZ2-E with the HLA-A1 molecule. Other members of the MAGE gene family do not code for the same peptide, suggesting that only MAGE-1 produces the antigen recognized by the anti-MZ2-E CTL. Our results open the possibility of immunizing HLA-A1 patients whose tumor expresses MAGE-1 either with the antigenic peptide or with autologous antigen-presenting cells pulsed with the peptide.

• Authors: C Traversari, P van der Bruggen, I F Luescher, C Lurquin, P Chomez, A Van Pel, E De Plaen, A Amar-Costesec, T Boon
• Journal: The Journal of experimental medicine (J Exp Med) Vol. 176 Issue 5 Pg. 1453-7 (Nov 01 1992) ISSN: 0022-1007 [Print] United States
• PMID: 1402688 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• HLA-A1 Antigen
• Oligopeptides

Topics

• Amino Acid Sequence
• Antigens, Neoplasm (genetics)
• Base Sequence
• HLA-A1 Antigen (immunology)
• Humans
• Melanoma (immunology)
• Molecular Sequence Data
• Oligopeptides (analysis)
• T-Lymphocytes, Cytotoxic (immunology)