Abstract |
Hemodilution has had limited success as a treatment of cerebral ischemia. When using a nonoxygen binding fluid, the therapeutic efficacy of hemodilution-induced increases in CBF are offset by concomitant decreases in oxygen content. The effect of hemodilution, with diaspirin alpha-alpha cross-linked hemoglobin ( DCLHb), on CBF during middle cerebral artery occlusion was assessed. Rats were hemodiluted to one of the following hematocrits (Hct): (a) 44/Hct, (b) 37/Hct, (c) 30/Hct, (d) 23/Hct, (e) 16/Hct, or (f) 9/Hct. After 10 min of ischemia, CBF was determined with 14C-iodoantipyrine. Coronal brain sections were evaluated for areas with a CBF of 0-10 and 11-20 ml 100 g-1 min-1. In addition, oxygen delivery was calculated. In the center of the ischemic zone, both areas of low CBF were less in the 30/Hct, 23/Hct, and 16/Hct groups compared with the 44/Hct and 37/Hct groups; and both areas were less in the 9/Hct group compared with the other five groups (p < 0.05). For the hemisphere contralateral to occlusion, there was a direct correlation between hematocrit and oxygen delivery. However, for the hemisphere ipsilateral to occlusion, oxygen delivery increased as hematocrit decreased (44/Hct, 8.6 +/- 0.3 vs. 9/Hct, 13.6 +/- 0.4 [mean +/- SD, ml 100 g-1 min-1]). The results of this study support a hypothesis that hemodilution with DCLHb decreases the extent of focal cerebral ischemia.
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Authors | D J Cole, R M Schell, R J Przybelski, J C Drummond, K Bradley |
Journal | Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
(J Cereb Blood Flow Metab)
Vol. 12
Issue 6
Pg. 971-6
(Nov 1992)
ISSN: 0271-678X [Print] United States |
PMID | 1400651
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Cross-Linking Reagents
- Hemoglobins
- bis(3,5-dibromosalicyl)fumarate
- Aspirin
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Topics |
- Animals
- Aspirin
(analogs & derivatives, pharmacology)
- Brain
(blood supply)
- Brain Ischemia
(drug therapy)
- Cerebrovascular Circulation
(drug effects)
- Cross-Linking Reagents
(pharmacology)
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Hemodilution
- Hemoglobins
(chemistry, therapeutic use)
- Male
- Rats
- Rats, Inbred SHR
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