Triflavin, an Arg-Gly-Asp-containing antiplatelet peptide inhibits cell-substratum adhesion and melanoma cell-induced lung colonization.

Abstract

Triflavin, an Arg-Gly-Asp (RGD) containing peptide purified from Trimeresurus flavoviridis snake venom, inhibits human platelet aggregation by blocking fibrinogen binding to fibrinogen receptors associated with glycoprotein IIb/IIIa complex. In this study, we show that triflavin (1-30 micrograms/mouse) inhibits B16-F10 melanoma cell-induced lung colonization in C57BL/6 mice in a dose-dependent manner. In vitro, triflavin dose-dependently inhibits adhesion of B16-F10 melanoma cells to extracellular matrices (ECMs; i.e., fibronectin, fibrinogen, vitronectin, and collagen type I). Triflavin is approximately 600-800 times more potent than GRGDS at inhibiting cell adhesion. In addition, triflavin dose-dependently inhibits B16-F10 cell-induced platelet aggregation. These results imply that the inhibitory effect of triflavin on the adhesion of tumor cells to ECMs (e.g., fibronectin, vitronectin and collagen type I) and/or tumor cell-induced platelet aggregation may be partially responsible for its antimetastatic activity in C57BL/6 mice.

• Authors: J R Sheu, C H Lin, J L Chung, C M Teng, T F Huang
• Journal: Japanese journal of cancer research : Gann (Jpn J Cancer Res) Vol. 83 Issue 8 Pg. 885-93 (Aug 1992) ISSN: 0910-5050 [Print] Japan
• PMID: 1399825 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Crotalid Venoms
• Peptides
• Platelet Aggregation Inhibitors
• triflavin
• Thymidine

Topics

• Animals
• Antineoplastic Agents (pharmacology)
• Cell Adhesion (drug effects)
• Cell Survival (drug effects)
• Crotalid Venoms (pharmacology)
• DNA Replication (drug effects)
• Kinetics
• Lung Neoplasms (prevention & control, secondary)
• Melanoma, Experimental (blood, pathology, prevention & control)
• Mice
• Mice, Inbred C57BL
• Peptides (pharmacology)
• Platelet Aggregation (drug effects)
• Platelet Aggregation Inhibitors (pharmacology)
• Thymidine (metabolism)
• Tumor Cells, Cultured