Renal tubular epithelial antigen-containing immune complexes stimulate interleukin-1 production by monocytes from patients with glomerulonephritis.

Abstract

We have investigated the effect of immune complexes (IC) derived from human renal tubular epithelial (RTE) antigen on the release of interleukin-1 (IL-1) in monocyte cultures from patients with glomerulonephritis (GN). When peripheral blood monocytes (PBM) were activated by IC, substantial amounts of IL-1 could be detected in the supernatants as measured by mouse thymocyte assay. The IC-induced IL-1 activity was significantly higher in patients with GN than in normal controls. To avoid the effect of prostaglandins on the IL-1 assay, we cultured PBM with addition of indomethacin and assayed IL-1 activity in the culture supernatants. This cyclooxygenase inhibitor augmented IC-induced IL-1 production. The results suggest that IC are involved in stimulating IL-1 production by PBM and thus play a role in the immune response in GN.

• Authors: K Matsumoto
• Journal: International urology and nephrology (Int Urol Nephrol) Vol. 24 Issue 3 Pg. 319-26 ( 1992) ISSN: 0301-1623 [Print] Netherlands
• PMID: 1399388 (Publication Type: Journal Article)

Chemical References

• Antigen-Antibody Complex
• Antigens
• Interleukin-1
• renal tubular antigen
• Indomethacin

Topics

• Adolescent
• Adult
• Antigen-Antibody Complex (immunology)
• Antigens (immunology)
• Female
• Glomerulonephritis (immunology, metabolism)
• Humans
• Indomethacin (pharmacology)
• Interleukin-1 (biosynthesis)
• Kidney Tubules (immunology)
• Male
• Middle Aged
• Monocytes (drug effects, metabolism)