Abstract | OBJECTIVE: To determine if bronchoalveolar lavage fluid is a more potent antioxidant after acute lung injury in a sheep model compared with the baseline condition. DESIGN: Nonrandomized, controlled study, with repeated measures. SETTING: University research laboratory. SUBJECTS: Seven healthy adult sheep (25 to 50 kg) were studied with five experimental sheep and two control sheep. INTERVENTIONS: Sheep with lung-lymph fistulas were used to study the antioxidant activity of serum, lymph, and bronchoalveolar lavage fluid, both at baseline and after the iv infusion of endotoxin and subsequent induction of acute lung injury. Antioxidant activity was measured, and it reflects the ability of serum, lymph, and bronchoalveolar lavage fluid to inhibit lipid peroxidation. MEASUREMENTS AND MAIN RESULTS: When compared at several volumes, bronchoalveolar lavage fluid after acute lung injury was a more potent inhibitor of lipid peroxidation than bronchoalveolar lavage fluid at baseline. In contrast, antioxidant activity in both serum (69.6 +/- 4.5% vs. 47.2 +/- 4.6%; p = .001) and lymph (45.0 +/- 2.3% vs. 31.9 +/- 1.2%; p = .001) decreased with acute lung injury. CONCLUSIONS:
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Authors | E R Pacht, G C Kindt, M G Lykens |
Journal | Critical care medicine
(Crit Care Med)
Vol. 20
Issue 10
Pg. 1441-7
(Oct 1992)
ISSN: 0090-3493 [Print] United States |
PMID | 1395666
(Publication Type: Journal Article)
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Chemical References |
- Antioxidants
- Blood Proteins
- Endotoxins
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Topics |
- Animals
- Antioxidants
(analysis, metabolism, pharmacology)
- Blood Proteins
(pharmacokinetics)
- Bronchoalveolar Lavage Fluid
(chemistry, metabolism)
- Disease Models, Animal
- Endotoxins
- Evaluation Studies as Topic
- Female
- Lipid Peroxidation
(drug effects)
- Lymph
(chemistry)
- Male
- Pulmonary Gas Exchange
- Respiratory Distress Syndrome
(blood, etiology, metabolism)
- Sheep
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