1. The effects of two
platelet-activating factor (PAF) antagonists,
WEB 2086 and
BN 52021, in reducing the changes in extravasation (
Evans blue technique) and blood flow (radiolabelled
microsphere method) to various organs and tissues following
anaphylactic shock in the Nippostrongylus brasiliensis-sensitized rat were investigated. 2. Both antagonists attenuated
anaphylaxis-induced increases in
plasma protein leak in the trachea, stomach and small intestine, although they did not block extravasation in the colon and kidneys. 3.
Anaphylaxis-induced decreases in blood flow to the adrenals were effectively antagonized by
WEB 2086, although this antagonist did not reverse blood flow decreases to any other tissues.
BN 52021, on the other hand, did not alter
anaphylaxis-induced decreases in blood flow to the adrenals, but effectively prevented dramatic decreases in blood flow to the large and small bowel and spleen. 4.
Anaphylactic shock produced marked reduction in blood pressure that was partly reversed by
WEB 2086, whereas
BN 52021 effectively blocked the decreases in cardiac output. 5. Thus, PAF is responsible for some of the haemodynamic and extravasation of
protein changes associated with systemic
anaphylaxis in the rat, although the differential inhibition observed with the two antagonists suggests that PAF alters vascular responsiveness through different mechanisms in selected tissues.