Abstract |
Tentoxin is a naturally occurring phytotoxic peptide that causes seedling chlorosis and arrests growth in sensitive plants and algae. In vitro, it inhibits activity of the beta subunit of the plastid proton- adenosine triphosphatase ( ATPase) from sensitive species. Plastid atpB genes from six closely related, tentoxin-sensitive or -resistant Nicotiana species differ at codon 83, according to their response to the toxin: glutamate correlated with resistance and aspartate correlated with sensitivity. The genetic relevance of this site was confirmed in Chlamydomonas reinhardtii by chloroplast transformation. The alga, normally tentoxin-resistant, was rendered tentoxin-sensitive by mutagenesis of its plastid atpB gene at codon 83. Codon 83 may represent a critical site on the beta subunit that does not compete with nucleotide binding or other catalytic activities.
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Authors | A Avni, J D Anderson, N Holland, J D Rochaix, Z Gromet-Elhanan, M Edelman |
Journal | Science (New York, N.Y.)
(Science)
Vol. 257
Issue 5074
Pg. 1245-7
(Aug 28 1992)
ISSN: 0036-8075 [Print] United States |
PMID | 1387730
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Mycotoxins
- Peptides, Cyclic
- Adenosine Triphosphate
- Proton-Translocating ATPases
- tentoxin
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Topics |
- Adenosine Triphosphate
(biosynthesis)
- Amino Acid Sequence
- Animals
- Cell Division
(drug effects)
- Chlamydomonas
- Chloroplasts
(drug effects)
- Dose-Response Relationship, Drug
- Drug Resistance
(genetics)
- Hydrolysis
(drug effects)
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mycotoxins
(pharmacology)
- Peptides, Cyclic
(pharmacology)
- Plants, Toxic
- Proton-Translocating ATPases
(genetics, physiology)
- Rhodospirillum rubrum
- Sequence Homology, Nucleic Acid
- Tobacco
- Transformation, Genetic
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