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Tentoxin sensitivity of chloroplasts determined by codon 83 of beta subunit of proton-ATPase.

Abstract
Tentoxin is a naturally occurring phytotoxic peptide that causes seedling chlorosis and arrests growth in sensitive plants and algae. In vitro, it inhibits activity of the beta subunit of the plastid proton-adenosine triphosphatase (ATPase) from sensitive species. Plastid atpB genes from six closely related, tentoxin-sensitive or -resistant Nicotiana species differ at codon 83, according to their response to the toxin: glutamate correlated with resistance and aspartate correlated with sensitivity. The genetic relevance of this site was confirmed in Chlamydomonas reinhardtii by chloroplast transformation. The alga, normally tentoxin-resistant, was rendered tentoxin-sensitive by mutagenesis of its plastid atpB gene at codon 83. Codon 83 may represent a critical site on the beta subunit that does not compete with nucleotide binding or other catalytic activities.
AuthorsA Avni, J D Anderson, N Holland, J D Rochaix, Z Gromet-Elhanan, M Edelman
JournalScience (New York, N.Y.) (Science) Vol. 257 Issue 5074 Pg. 1245-7 (Aug 28 1992) ISSN: 0036-8075 [Print] United States
PMID1387730 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Mycotoxins
  • Peptides, Cyclic
  • Adenosine Triphosphate
  • Proton-Translocating ATPases
  • tentoxin
Topics
  • Adenosine Triphosphate (biosynthesis)
  • Amino Acid Sequence
  • Animals
  • Cell Division (drug effects)
  • Chlamydomonas
  • Chloroplasts (drug effects)
  • Dose-Response Relationship, Drug
  • Drug Resistance (genetics)
  • Hydrolysis (drug effects)
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Mycotoxins (pharmacology)
  • Peptides, Cyclic (pharmacology)
  • Plants, Toxic
  • Proton-Translocating ATPases (genetics, physiology)
  • Rhodospirillum rubrum
  • Sequence Homology, Nucleic Acid
  • Tobacco
  • Transformation, Genetic

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