Co-ordinate expression of c-fos, p53 and cytokeratin genes during the alteration of growth of human hepatoma cells. mRNA levels measured by reverse transcription and polymerase chain reaction.

Abstract

Teleocidin, a tumor promoter, inhibited the proliferation, enhanced cytokeratin assembly and increased the type III procollagen production of PLC/PRF/5 hepatoma cells. Teleocidin transiently increased the levels of c-fos and p53 mRNAs measured by reverse transcription and polymerase chain reaction. This was followed by a reduction of c-myc mRNA and an increase of cytokeratin mRNA. The level of p120 mRNA was not remarkably altered. Sequential alterations of the expression of c-fos, p53, c-myc and cytokeratin genes induced by teleocidin may be responsible for the morphological and functional changes of hepatoma cells induced by this tumor promoter.

• Authors: Y Kaneko, A Tsukamoto, K Korokawa
• Journal: Cancer letters (Cancer Lett) Vol. 66 Issue 2 Pg. 155-64 (Sep 30 1992) ISSN: 0304-3835 [Print] Ireland
• PMID: 1382834 (Publication Type: Journal Article)

Chemical References

• Lyngbya Toxins
• RNA, Messenger
• teleocidins
• Keratins
• RNA-Directed DNA Polymerase

Topics

• Base Sequence
• Carcinoma, Hepatocellular (metabolism, pathology)
• Gene Expression
• Genes, fos
• Genes, myc
• Genes, p53
• Humans
• Keratins (genetics)
• Liver Neoplasms (metabolism, pathology)
• Lyngbya Toxins (pharmacology)
• Molecular Sequence Data
• Polymerase Chain Reaction
• RNA, Messenger (analysis)
• RNA-Directed DNA Polymerase (genetics)
• Tumor Cells, Cultured