[Possibility of selective binding of HIV-1 infected cells by immunosorbents].

Abstract

A study was made of a possibility of specific sorption of HIV-1 infected cells with gamma-fraction of AIDS patients' serum containing high titer-specific antibodies, immobilized on the silica matrix (C-3). The laboratory tests were made with the use of the monocyte culture [symbol: see text] chronically infected with HIV-1. In addition to a decrease of the cell count after sorption, there was a decline of the activity of antigen material in the samples (to 43% of the initial). The selectivity rates were 0.92 for immunosorbents and 0.29 for sorbents of the control group, that confirms and increase of the delay of the cells expressing HIV-1 proteins on a column with the immobilized gamma-fraction of AIDS patients' serum. The use of the synthesized immunosorbents in vivo may appear debatable. However, this method can be employed for preparative purposes.

• Authors: Iu M Lopukhin, V V Pavlenko, D V Kulaev, V S Alekseĭchuk, E V Karamov, I A Rudneva, D D Petrunin, T K Liukova
• Journal: Vestnik Rossiiskoi akademii meditsinskikh nauk (Vestn Ross Akad Med Nauk) Issue 3 Pg. 54-6 ( 1992) ISSN: 0869-6047 [Print] Russia (Federation)
• Vernacular Title: Izuchenie vozmozhnosti selektivnogo sviazyvaniia infitsirovannykh VICh-1 kletok s pomoshch'iu immunosorbentov.
• PMID: 1382761 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• HIV Antibodies
• Immunosorbents
• Viral Proteins

Topics

• Acquired Immunodeficiency Syndrome (immunology, metabolism)
• Cell Count
• Cells, Cultured
• Enzyme-Linked Immunosorbent Assay
• Fluorescent Antibody Technique
• HIV Antibodies (analysis)
• HIV-1 (immunology, metabolism)
• Humans
• Immunosorbents
• Viral Proteins (metabolism)