Transcriptional regulation of Bacillus subtilis glucose starvation-inducible genes: control of gsiA by the ComP-ComA signal transduction system.

Abstract	The Bacillus subtilis glucose starvation-inducible transcription units, gsiA and gsiB, were characterized by DNA sequencing, transcriptional mapping, mutational analysis, and expression in response to changes in environmental conditions. The gsiA operon was shown to consist of two genes, gsiAA and gsiAB, predicted to encode 44.9- and 4.8-kDa polypeptides, respectively. The gsiB locus contains a single cistron which encodes a protein of unusual structure; most of its amino acids are arranged in five highly conserved, tandemly repeated units of 20 amino acids. The 5' ends of gsiA and gsiB mRNAs were located by primer extension analysis; their locations suggest that both are transcribed by RNA polymerase containing sigma A. Expression of both gsiA and gsiB was induced by starvation for glucose or phosphate or by addition of decoyinine, but only gsiA was induced by exhaustion of nutrient broth or by amino acid starvation. Regulation of gsiA expression was shown to be dependent upon the two-component signal transduction system ComP-ComA, which also controls expression of genetic competence genes. Mutations in mecA bypassed the dependency of gsiA expression on ComA. Disruption of gsiA relieved glucose repression of sporulation but did not otherwise interfere with sporulation, development of competence, motility, or glucose starvation survival. We propose that gsiA and gsiB are members of an adaptive pathway of genes whose products are involved in responses to nutrient deprivation other than sporulation.
Authors	J P Mueller, G Bukusoglu, A L Sonenshein
Journal	Journal of bacteriology (J Bacteriol) Vol. 174 Issue 13 Pg. 4361-73 (Jul 1992) ISSN: 0021-9193 [Print] United States
PMID	1378051 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References	Bacterial Proteins ComA protein, Bacteria DNA, Bacterial DNA-Binding Proteins Membrane Proteins RNA, Bacterial RNA, Messenger Recombinant Fusion Proteins rapA protein, Bacillus subtilis gsiB protein, Bacillus subtilis Transferases comP protein, Bacillus subtilis Phosphoprotein Phosphatases beta-Galactosidase Glucose
Topics	Amino Acid Sequence Bacillus subtilis (genetics, growth & development, metabolism) Bacterial Proteins (genetics, metabolism) Base Sequence Cloning, Molecular DNA, Bacterial (genetics) DNA-Binding Proteins Gene Expression Regulation, Bacterial Genes, Bacterial Genotype Glucose (metabolism) Membrane Proteins Molecular Sequence Data Phosphoprotein Phosphatases Plasmids Promoter Regions, Genetic RNA, Bacterial (genetics, isolation & purification) RNA, Messenger (genetics, metabolism) Recombinant Fusion Proteins Restriction Mapping Sequence Homology, Nucleic Acid Signal Transduction Transcription, Genetic Transferases beta-Galactosidase (genetics, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!