Abstract |
The six hour peritoneal lavage with glutaryl-trialanin-ethylamide, a low molecular competitive inhibitor of pancreatic elastase (IC50-8 mumol/l), effectively suppresses the evolution of taurocholate induced acute pancreatitis in the rat. The lavage alone is followed by a marked decrease of fat necrosis and amylase and lipase activity in serum. The area of pancreatic haemorrhage was significantly reduced only after the lavage solution was supplemented with Glt-Ala3-NHEt. The effect was not enhanced by a bolus injection of the inhibitor before starting the lavage. The combination of Glt-Ala3-NHEt with aprotinin or nafamstate mesilate produced only marginal greater benefit. The effect of Glt-Ala3-NHEt on pancreatic haemorrhage is time and dose related even with delayed onset of the lavage. Animals treated with peritoneal lavage without Get-Ala3-NHEt lived longer than controls (p less than 0.05), but by 60 hours the survival rate of both groups was almost the same (76 v 74%). All animals lavaged with Glt-Ala3-NHEt survived 120 hours and the difference in the survival rate between this and both remaining groups was significant (100% v 76% v 74% - p less than 0.05). The results were considered favourable and preliminary clinical trials of Glt-Ala3-NHEt in subjects with acute pancreatitis justified.
|
Authors | P Fric, J Slabý, E Kasafírek, P Kocna, J Marek |
Journal | Gut
(Gut)
Vol. 33
Issue 5
Pg. 701-6
(May 1992)
ISSN: 0017-5749 [Print] England |
PMID | 1377154
(Publication Type: Journal Article)
|
Chemical References |
- Oligopeptides
- Taurocholic Acid
- glutaryl-alanyl-alanyl-alanyl-ethylamide
- Lipase
- Amylases
- Pancreatic Elastase
|
Topics |
- Amylases
(blood)
- Animals
- Dose-Response Relationship, Drug
- Fat Necrosis
(prevention & control)
- Lipase
(blood)
- Male
- Oligopeptides
(therapeutic use)
- Pancreatic Elastase
(antagonists & inhibitors)
- Pancreatitis
(chemically induced, drug therapy, enzymology)
- Peritoneal Lavage
- Rats
- Rats, Inbred Strains
- Taurocholic Acid
- Time Factors
|