Structural determination of gangliosides that bind to influenza A, B, and C viruses by an improved binding assay: strain-specific receptor epitopes in sialo-sugar chains.

Abstract

An improved binding assay for detection of ganglioside receptors for influenza A, B, and C viruses was developed. In this system, the virions bound to gangliosides that were developed on a silica gel thin-layer plate were detected by mouse monoclonal antibody against viral hemagglutinin and peroxidase-conjugated anti-mouse immunoglobin. No hydrolysis of the gangliosides by viral receptor-destroying enzyme was detected in the present condition. The reactivity of the viruses to gangliosides depended on the amount of developed gangliosides (10 pmols-10 nmols), the molecular species of sialic acid, and their sugar sequences. Human influenza A (PR/8/34), B (Lee/40), and C (Ann Arbor/1/50) viruses bound different receptor epitopes of sialo-sugar chains of gangliosides. The A/PR/8 virus bound most effectively to Neu5Ac-containing lacto-series gangliosides carrying type I and type II sugar chains, followed by ganglio-series and hematoside-series gangliosides. The A/PR/8 virus weakly bound to Neu5Ac alpha 2,6lactotetraosylceramide [IV6(Neu5Ac)Lc4Cer] and Neu5Ac alpha 2,6paragloboside [IV6(Neu5Ac)nLc4Cer] carrying Neu5Ac alpha 2,6Gal sequence, although their Neu5Ac alpha 2,3Gal derivatives were the most potent gangliosides tested. B/Lee/40 bound restrictively to IV6(Neu5Ac)Lc4Cer and IV6(Neu5Ac)nLc4Cer, which carry Neu5Ac alpha 2,6Gal sequence, and type I and type II lacto-series sugar chain, respectively. C/Ann Arbor/1/50 reacted only with 9-O-Ac-Neu5Ac-carrying sugar chains in all the gangliosides tested. This method also allowed the microanalysis of receptor gangliosides of unknown samples. ESK cells, sensitive to the influenza A viruses infection, expressed several kinds of receptor active gangliosides, while those from ESK-R cells, resistant to the virus infection, were undetectable.

• Authors: Y Suzuki, T Nakao, T Ito, N Watanabe, Y Toda, G Xu, T Suzuki, T Kobayashi, Y Kimura, A Yamada
• Journal: Virology (Virology) Vol. 189 Issue 1 Pg. 121-31 (Jul 1992) ISSN: 0042-6822 [Print] United States
• PMID: 1376537 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Epitopes
• Gangliosides
• Globosides
• Oligosaccharides
• Receptors, Virus
• sialooligosaccharides
• sialosylparagloboside

Topics

• Binding Sites
• Carbohydrate Sequence
• Epitopes (immunology, metabolism)
• Gangliosides (chemistry, metabolism)
• Globosides (metabolism)
• Immunologic Techniques
• Influenza A virus (immunology, metabolism)
• Influenza B virus (immunology, metabolism)
• Influenzavirus C (immunology, metabolism)
• Molecular Sequence Data
• Oligosaccharides (metabolism)
• Orthomyxoviridae (immunology, metabolism)
• Receptors, Virus (chemistry, metabolism)
• Sensitivity and Specificity
• Virology (methods)