Activated lymphocyte subsets in adult periodontitis.

Abstract	The activation state of T and B lymphocytes in the peripheral blood of periodontitis patients may be a reflection of disease activity. We have utilized 2- and 3-color flow cytometric analyses using a new chromophore, peridinin chlorophyll A protein, and conventional dyes, fluorescein isothiocyanate and phycoerythrin, conjugated to monoclonal antibodies against activated lymphocyte surface markers to measure blood lymphocyte subsets from 18 periodontitis patients and 16 periodontally healthy control subjects. Two-color flow cytometric analysis demonstrated that the frequency of CD4+ and CD5+ T cells, CD20+ B cells, and CD16+ NK (natural killer) cells were increased in periodontitis patients. Of particular interest, CD4+ activated "memory" T cells, CD5+ B cells, and CD56+ NK effector cells were increased significantly in periodontitis patients (p less than 0.05). While the relationship of lymphocyte activation to periodontal disease activity remains unclear, there may be potential for using 2- and 3-color flow cytometry to subcategorize periodontitis patients into high- and moderate-risk groups.
Authors	B Afar, D Engel, E A Clark
Journal	Journal of periodontal research (J Periodontal Res) Vol. 27 Issue 2 Pg. 126-33 (Mar 1992) ISSN: 0022-3484 [Print] United States
PMID	1372351 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References	Antibodies, Monoclonal Antigens, CD Antigens, CD20 Antigens, Differentiation Antigens, Differentiation, B-Lymphocyte Antigens, Differentiation, T-Lymphocyte CD3 Complex CD4 Antigens CD5 Antigens CD56 Antigen Receptors, Antigen, T-Cell Receptors, Fc Receptors, IgG Phycoerythrin peridinin Carotenoids Fluorescein-5-isothiocyanate
Topics	Adult Aged Antibodies, Monoclonal Antigens, CD (blood) Antigens, CD20 Antigens, Differentiation (analysis) Antigens, Differentiation, B-Lymphocyte (blood) Antigens, Differentiation, T-Lymphocyte (blood) CD3 Complex CD4 Antigens (blood) CD5 Antigens CD56 Antigen Carotenoids Female Flow Cytometry Fluorescein-5-isothiocyanate Humans Killer Cells, Natural Lymphocyte Activation Lymphocyte Subsets Male Middle Aged Periodontitis (blood, immunology) Phycoerythrin Receptors, Antigen, T-Cell (metabolism) Receptors, Fc (analysis) Receptors, IgG T-Lymphocytes, Helper-Inducer T-Lymphocytes, Regulatory

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